产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
CD45抗体(C末端)
目录 :
MBS9202938
规格 :
0.08毫升
价格 :
165美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪, 流式细胞仪, 酶免疫法
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS9202938 图像 1
The anti-CD45 (C-term) Pab (Cat. # MBS9202938) is used in Western blot to detect CD45 in jurkat cell lysate.
图像 2 :
MyBioSource MBS9202938 图像 2
Formalin-fixed and paraffin-embedded human tonsil reacted with CD45 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
图像 3 :
MyBioSource MBS9202938 图像 3
Flow cytometric analysis of jurkat cells using CD45 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
产品信息
目录号 :
MBS9202938
产品类型 :
抗体
产品全称 :
CD45抗体(C末端)
产品简称 :
[CD45]
产品名称同义词 :
[受体类型酪氨酸蛋白磷酸酶C;白细胞共同抗原;L-CA;T200;CD45;蛋白酪氨酸磷酸酶受体C;CD45]
其他名称 :
[receptor-type tyrosine-protein phosphatase C isoform 1; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C; protein tyrosine phosphatase, receptor type, C; Leukocyte common antigen; L-CA; T200; CD_antigen: CD45]
产品基因名称 :
[蛋白酪氨酸磷酸酶受体C]
其他基因名称 :
[蛋白酪氨酸磷酸酶受体C;蛋白酪氨酸磷酸酶受体C;LCA;LY5;B220;CD45;L-CA;T200;CD45R;GP180;CD45;L-CA]
UniProt数据库进入名 :
PTPRC_HUMAN
克隆性 :
多克隆
抗体亚型 :
兔IgE
宿主 :
反应物种 :
人类
序列位置 :
[1245-1275]
序列长度 :
1306
特异性 :
This CD45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1245-1275 amino acids from the C-terminal region of human CD45.
纯度 :
纯化兔多克隆抗体(多克隆抗体)
形式 :
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
浓度 :
Vial Concentration: 2
储存稳定性 :
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
检测过的应用 :
免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA), 免疫组化(免疫组化), 流式细胞仪(FC/流式细胞仪)
应用笔记 :
WB~~1:1000。 免疫组化-P~~1:10~50。 FC~~1:10~50
image1头 :
免疫印迹
image2头 :
免疫组化
image3头 :
流式细胞仪
其它信息1 :
Antigen Type: Synthetic Peptide. Clone Names: RB00430
其他信息2 :
Antigen Source: HUMAN. Function: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Cellular Location: Membrane; Single-pass type I membrane protein Membrane raft. Note=Colocalized with DPP4 in membrane rafts
产品种类 :
Cancer; Immunology; Signal Transduction
产品描述 :
CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. The CD45 gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.
产品引用 :
Stanton, T., et al., Proc. Natl. Acad. Sci. U.S.A. 100(10):5997-6002 (2003). Vogel, A., et al., Genes Immun. 4(1):79-81 (2003). Rachmilewitz, J., et al., J. Biol. Chem. 278(16):14059-14065 (2003). McCann, F.E., et al., J. Immunol. 170(6):2862-2870 (2003). Fernandis, A.Z., et al., J. Biol. Chem. 278(11):9536-9543 (2003).
NCBI GI登录号 :
392307007
NCBI登录号 :
NP_002829.3
NCBI基因登录号 :
NM_002838.4
UniProt数据库登录号 :
P08575
NCBI分子量 :
147254
NCBI信号通路 :
甲烷代谢通路(5788);甲烷代谢通路(5788);甲烷代谢通路(5788);甲烷代谢通路(5788);甲烷代谢通路(5788);甲烷代谢通路(5788);甲烷代谢通路(5788);甲烷代谢通路(5788);甲烷代谢通路(5788);甲烷代谢通路(5788)
NCBI总结 :
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
UniProt数据库总结 :
CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described. Protein type: Membrane protein, integral; Receptor protein phosphatase, tyrosine; EC 3.1.3.48; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q31-q32. Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane; lipid raft. Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding. Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; positive regulation of antigen receptor-mediated signaling pathway; T cell receptor signaling pathway; bone marrow development; B cell receptor signaling pathway; stem cell development; cell surface receptor linked signal transduction; dephosphorylation; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; positive regulation of T cell proliferation; hemopoietic progenitor cell differentiation; negative regulation of cytokine and chemokine mediated signaling pathway; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation. Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive; Hepatitis C Virus, Susceptibility To
尺寸1 :
0.08毫升
价格1 :
165美元
尺寸2 :
0.4毫升
价格2 :
370
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。