产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
CD19抗体(N末端)
目录 :
MBS9204578
规格 :
0.08毫升
价格 :
165美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 酶免疫法, 免疫组化-石蜡切片
更多信息或购买 :
MyBioSource 产品网页
图像
图像 1 :
MyBioSource MBS9204578 图像 1
Western blot analysis of lysate from Ramos cell line, using CD19Antibody (N-term). MBS9204578 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
图像 2 :
MyBioSource MBS9204578 图像 2
CD19 Antibody (N-term) western blot analysis in Raji cell line lysates (35ug/lane).This demonstrates the CD19 antibody detected the CD19 protein (arrow).
图像 3 :
MyBioSource MBS9204578 图像 3
Western blot analysis of CD19 (arrow) using rabbit polyclonal CD19 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2) (Origene Technologies).
产品信息
目录号 :
MBS9204578
产品类型 :
抗体
产品全称 :
CD19抗体(N末端)
产品简称 :
[CD19]
产品名称同义词 :
[B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19; CD19]
其他名称 :
[B-lymphocyte antigen CD19 isoform 1; B-lymphocyte antigen CD19; B-lymphocyte antigen CD19; CD19 molecule; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19]
产品基因名称 :
[CD19]
其他基因名称 :
[CD19;CD19;B4;CVID3]
UniProt数据库进入名 :
CD19_HUMAN
克隆性 :
多克隆
抗体亚型 :
兔IgE
宿主 :
兔
反应物种 :
人类
序列位置 :
[143-172]
序列长度 :
557
特异性 :
This CD19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the N-terminal region of human CD19.
纯度 :
肽亲和纯化兔多克隆抗体(多克隆抗体)
形式 :
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
储存稳定性 :
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
检测过的应用 :
免疫印迹(免疫印迹), 免疫荧光(IF), 流式细胞仪(FC), 免疫组化石蜡(免疫组化-P), 酶联免疫吸附测定(EIA)
应用笔记 :
WB~~1:1000。 IF~~1:10~50。 FC~~1:10~50。 免疫组化-P~~1:10~50
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫印迹(免疫印迹)
image3头 :
免疫印迹(免疫印迹)
image4头 :
免疫荧光(IF)
image4描述 :
Immunofluorescence analysis of CD19 Antibody (N-term) with paraffin-embedded human lymph tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
image5头 :
流式细胞仪(FC/流式细胞仪)
image5描述 :
Flow cytometric analysis of CEM cells using CD19 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
其他信息2 :
Function: Assembles with the antigen receptor ofB-lymphocytes in order to decrease thethreshold for antigen receptor-dependentstimulation. Cellular Location: Membrane; Single-pass type I membrane protein
产品种类 :
Crown Antibodies; Cancer; Immunology; Signal Transduction
产品描述 :
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
产品引用 :
Deaglio,S., Blood 109 (12), 5390-5398 (2007). Bradbury,L.E., J. Immunol. 149 (9), 2841-2850 (1992). Kozmik,Z., Mol. Cell. Biol. 12 (6), 2662-2672 (1992)
NCBI GI登录号 :
296010921
NCBI登录号 :
NP_001171569.1
NCBI基因登录号 :
NM_001178098.1
UniProt数据库登录号 :
P15391
NCBI总结 :
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
CD19: a cell surface molecule which assembles with the antigen receptor of B lymphocytes. A critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. Ligation increases intracellular calcium levels and decreases the threshold for antigen receptor signaling. Germinal center formation is significantly reduced in CD19-deficient mice. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: protein complex; integral to plasma membrane; plasma membrane; external side of plasma membrane. Molecular Function: receptor signaling protein activity. Biological Process: epidermal growth factor receptor signaling pathway; regulation of immune response; B cell receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cell surface receptor linked signal transduction; nerve growth factor receptor signaling pathway; cellular defense response; innate immune response; positive regulation of release of sequestered calcium ion into cytosol. Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 3
尺寸1 :
0.08毫升
价格1 :
165美元
尺寸2 :
0.4毫升
价格2 :
370
更多信息或购买 :
MyBioSource 产品网页
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。