抗体

SPARC抗体(C末端)

MBS9207505

0.08毫升

165美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠, 鸡

免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪, 流式细胞仪, 酶免疫法, 免疫组化-石蜡切片

抗体

SPARC抗体(C末端)

[SPARC]

[SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine; SPARC; ON]

[SPARC isoform 1; SPARC; SPARC; secreted protein, acidic, cysteine-rich (osteonectin); Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine]

[SPARC]

[SPARC;SPARC;ON;BM-40;ON;BM-40;ON]

SPRC_HUMAN

多克隆

兔IgE

兔

Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus)

[224-251]

303

This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.

纯化兔多克隆抗体(多克隆抗体)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA), 免疫组化(免疫组化-P), 流式细胞仪(FC/流式细胞仪)

WB~~1:1000。 免疫组化-P~~1:10~50。 FC~~1:10~50

免疫印迹(免疫印迹)

免疫印迹(免疫印迹)

免疫组化(免疫组化)

流式细胞仪(FC/流式细胞仪)

Flow cytometric analysis of widr cells using SPARC Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Function: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Cellular Location: Secreted, extracelluar matrix, basement membrane. Note= In or around the basement membrane.

Cancer; Cardiovascular; Cell Biology; Immunology

Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).

Said,N., Oncogene 28 (39), 3487-3498 (2009). Maloney,S.C., Anticancer Res. 29 (8), 3059-3064 (2009)

4507171

NP_003109.1

NM_003118.3

P09486

34632

Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678); Taurine And Hypotaurine Metabolism Pathway (6678)

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5q31.3-q32. Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; extracellular region; plasma membrane; basement membrane. Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding. Biological Process: response to gravity; response to peptide hormone stimulus; platelet activation; receptor-mediated endocytosis; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; response to cadmium ion; platelet degranulation; response to ethanol; response to cytokine stimulus; response to lead ion; negative regulation of endothelial cell proliferation; regulation of cell morphogenesis; blood coagulation; response to calcium ion; inner ear development; lung development. Disease: Osteogenesis Imperfecta, Type Xvii

0.08毫升

165美元

0.4毫升

370