抗体

小鼠Cdk9抗体(C末端)

MBS9208988

0.08毫升

165美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

小鼠Cdk9抗体(C末端)

[Cdk9]

[细胞周期蛋白依赖激酶9;细胞分裂蛋白激酶9;Cdk9]

[细胞周期蛋白依赖激酶9;细胞周期蛋白依赖激酶9;细胞周期蛋白依赖激酶9;细胞周期蛋白依赖激酶9(cdc2相关激酶);细胞分裂蛋白激酶9]

[Cdk9]

[Cdk9;Cdk9;PITALRE]

CDK9_MOUSE

多克隆

兔IgE

兔

Human, mouse (Predicted Reactivity: Bovine, Rat)

[251-278]

372

This Mouse Cdk9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-278 amino acids from the C-terminal region of mouse Cdk9.

肽亲和纯化兔多克隆抗体(多克隆抗体)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA)

WB~~1:1000

免疫印迹(免疫印迹)

Function: Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single- stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation (By similarity).

Cellular Location: Nucleus. Cytoplasm. Nucleus, PML body Note=Accumulates on chromatin in response to replication stress Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm. The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent. Associates with PML body when acetylated (By similarity). Tissue Location: Expressed at high levels in brain and kidney

牙冠抗体;细胞生物学;免疫学;神经科学;信号转导

Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro (By similarity).

Yokoyama, S., et al. Dev. Cell 17(6):836-848(2009). Alarcon, C., et al. Cell 139(4):757-769(2009). Takaya, T., et al. Circ. J. 73(8):1492-1497(2009). Kohoutek, J., et al. Mol. Cell. Biol. 29(12):3280-3285(2009). Elagib, K.E., et al. Blood 112(13):4884-4894(2008)

18699998

NP_570930.1

NM_130860.3

Q99J95

42762

通路(107951);, 通路(107951);, 通路(107951);, 通路(107951);, 通路(107951);, 通路(107951);, 通路(107951);, 通路(107951);, 通路(107951);, 通路(107951)

CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol. Protein type: EC 2.7.11.22; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.23; Nuclear receptor co-regulator; Cell cycle regulation; CMGC group; CDK family; CDK9 subfamily; CDK/CDK9 subfamily. Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; membrane; intracellular membrane-bound organelle; cytoplasm; nucleus. Molecular Function: nucleotide binding; transcription factor binding; protein kinase activity; RNA polymerase subunit kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; chromatin binding; kinase activity; ATP binding. Biological Process: regulation of DNA repair; regulation of histone modification; positive regulation of histone phosphorylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; replication fork arrest; DNA repair; protein amino acid phosphorylation; response to DNA damage stimulus; phosphorylation

0.08毫升

165美元

0.4毫升

370