抗体

BRAF抗体

MBS9209378

0.08毫升

165美元

多克隆

兔

未共轭

免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 流式细胞仪, 酶免疫法

抗体

BRAF抗体

[BRAF]

[Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1; RAFB1]

[serine/threonine-protein kinase B-raf; Serine/threonine-protein kinase B-raf; serine/threonine-protein kinase B-raf; B-Raf proto-oncogene, serine/threonine kinase; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1]

[BRAF]

[BRAF;BRAF;NS7;BRAF1;RAFB1;B-RAF1;BRAF1;RAFB1]

BRAF_HUMAN

多克隆

兔IgE

兔

人类

766

This BRAF antibody is generated from rabbits immunized with BRAF recombinant protein.

肽亲和纯化兔多克隆抗体(多克隆抗体)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

流式细胞仪(FC/流式细胞仪), 酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹)

WB~~1:1000。 FC~~1:10~50

免疫印迹(免疫印迹)

流式细胞仪(FC/流式细胞仪)

Function: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. Cellular Location: Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. Tissue Location: Brain and testis.

Crown Antibodies; Cancer; Cell Biology; Signal Transduction

BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

Hingorani, S.R., et al., Cancer Res. 63(17):5198-5202 (2003). Lee, J.W., et al., Br. J. Cancer 89(10):1958-1960 (2003). Davies, H., et al., Nature 417(6892):949-954 (2002). Naoki, K., et al., Cancer Res. 62(23):7001-7003 (2002). Stephens, R.M., et al., Mol. Cell. Biol. 12(9):3733-3742 (1992).

33188459

NP_004324.2

NM_004333.4

P15056

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]

BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib. Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Oncoprotein; EC 2.7.11.1; Protein kinase, TKL; TKL group; RAF family. Chromosomal Location of Human Ortholog: 7q34. Cellular Component: neuron projection; mitochondrion; plasma membrane; nucleus; cytosol. Molecular Function: small GTPase binding; protein serine/threonine kinase activity; identical protein binding; protein binding; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein heterodimerization activity; calcium ion binding; ATP binding; protein kinase activity. Biological Process: positive T cell selection; response to peptide hormone stimulus; response to cAMP; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; CD4-positive, alpha beta T cell differentiation; protein heterooligomerization; somatic stem cell maintenance; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; synaptic transmission; organ morphogenesis; myeloid progenitor cell differentiation; small GTPase mediated signal transduction; positive regulation of stress fiber formation; visual learning; negative regulation of neuron apoptosis; negative regulation of apoptosis. Disease: Noonan Syndrome 1; Noonan Syndrome 7; Lung Cancer; Leopard Syndrome 3; Cardiofaciocutaneous Syndrome 1

0.08毫升

165美元

0.4毫升

370