抗体

KRAS抗体(C末端)

MBS9210256

0.08毫升

165美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 流式细胞仪, 酶免疫法

抗体

KRAS抗体(C末端)

[KRAS]

[GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; GTPase KRas; N-terminally processed; KRAS; KRAS2; RASK2]

[GTPase KRas isoform b; GTPase KRas; GTPase KRas; Kirsten rat sarcoma viral oncogene homolog; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras]

[KRAS]

[KRAS;KRAS;NS;NS3;CFC2;KRAS1;KRAS2;RASK2;KI-RAS;C-K-RAS;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;KRAS2;RASK2]

RASK_HUMAN

多克隆

兔IgE

兔

人类, 小鼠, 大鼠

[146-174]

188

This KRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the C-terminal region of human KRAS.

肽亲和纯化兔多克隆抗体(多克隆抗体)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA), 免疫荧光(IF), 流式细胞仪(FC/流式细胞仪)

WB~~1:2000。 IF~~1:10~50。 FC~~1:10~50

免疫印迹(免疫印迹)

免疫印迹(免疫印迹)

免疫印迹(免疫印迹)

免疫荧光(IF)

Confocal immunofluorescent analysis of KRAS Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

流式细胞仪(FC/流式细胞仪)

KRAS Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Type: Synthetic Peptide

Antigen Source: HUMAN. Clone Names: RB32708. Function: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed: 23698361 , PubMed: 22711838 ). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer(CRC) cells in a ZNF304-dependent manner(PubMed: 24623306 ). Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol

Cancer; Signal Transduction

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Bruckman, K.C., et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110(5):632-637(2010). Wong, K.K., et al. Am. J. Pathol. 177(4):1611-1617(2010). Irahara, N., et al. Diagn. Mol. Pathol. 19(3):157-163(2010). Carotenuto, P., et al. Pharmacogenomics 11(8):1169-1179(2010). Leventopoulos, G., et al. Clin. Exp. Rheumatol. 28(4):556-557(2010)

15718761

NP_004976.2

NM_004985.4

P01116

21656

通路(3845);, 通路(3845);, 通路(3845);, 通路(3845);, 通路(3845);, 通路(3845);, 通路(3845);, 通路(3845);, 通路(3845);, 通路(3845)

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Ras; Oncoprotein. Chromosomal Location of Human Ortholog: 12p12.1. Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; membrane; mitochondrion; cytoplasm; plasma membrane; cytosol; lipid raft. Molecular Function: protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding. Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; nerve growth factor receptor signaling pathway; activation of MAPKK activity; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of nitric-oxide synthase activity; MAPKKK cascade; social behavior; response to mineralocorticoid stimulus; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; striated muscle cell differentiation; innate immune response; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; leukocyte migration. Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Breast Cancer; Lung Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2

0.08毫升

165美元

0.4毫升

370