ELISA/assay

大鼠乙类型血小板衍生生长因子受体, 血小板衍生的生长因子受体b酶联免疫吸附试剂盒

MBS928462

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

大鼠乙类型血小板衍生生长因子受体, 血小板衍生的生长因子受体b酶联免疫吸附试剂盒

platelet-derived growth factor receptor, beta polypeptide

Rat Beta-type platelet-derived growth factor receptor (PDGFRB) ELISA kit; CD140B; JTK12; PDGFR; PDGFR1; CD140 antigen-like family member B; PDGF-R-beta; platelet-derived growth factor receptor beta; platelet-derived growth factor receptor; beta polypeptide

platelet-derived growth factor receptor beta; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta; PDGFR-beta; PDGF-R-beta; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta platelet-derived growth factor receptor; Platelet-derived growth factor receptor, beta; beta-type platelet-derived growth factor receptor; platelet-derived growth factor receptor beta variant 1; platelet derived growth factor receptor, beta polypeptide; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD_antigen: CD140b

血小板衍生的生长因子受体b

Pdgfrb; Pdgfrb; PDGFR-1; Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1

PGFRB_RAT

大鼠

1097

This assay has high sensitivity and excellent specificity for detection of rat PDGFRB. No significant cross-reactivity or interference between rat PDGFRB and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 47 pg/ml -3000 pg/ml. Sensitivity: The minimum detectable dose of rat PDGFRB is typically less than 11.75 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGFRB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGFRB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFRB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFRB bound in the initial step. The color development is stopped and the intensity of the color is measured.

29789275

NP_113713.1

NM_031525.1

Q05030

122,828 Da

Calcium Signaling Pathway (83442); Calcium Signaling Pathway (459); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Downstream Signal Transduction Pathway (574194); Focal Adhesion Pathway (83459); Focal Adhesion Pathway (478); Gap Junction Pathway (83464); Gap Junction Pathway (483); Glioma Pathway (83498)

binds platelet derived growth factor BB homodimer [RGD, Feb 2006]

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent. Protein type: Kinase, protein; EC 2.7.10.1; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; PDGFR family

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800