ELISA/assay

人类层粘连蛋白亚单位甲3, LAMA3酶联免疫吸附试剂盒

MBS931822

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

人类层粘连蛋白亚单位甲3, LAMA3酶联免疫吸附试剂盒

层粘连蛋白, 甲3

Human Laminin subunit alpha-3 (LAMA3) ELISA kit; BM600; E170; LAMNA; LOCS; lama3a; BM600 150kD subunit; epiligrin 170 kda subunit; epiligrin alpha 3 subunit; kalinin 165kD subunit; laminin alpha 3 subunit; laminin; alpha 3 (nicein (150kD); kalinin (165; laminin; alpha 3

laminin subunit alpha-3 isoform 2; Laminin subunit alpha-3; laminin subunit alpha-3; BM600 150kD subunit; nicein 150kD subunit; nicein subunit alpha; kalinin 165kD subunit; kalinin subunit alpha; epiligrin subunit alpha; laminin-5 alpha 3 chain; laminin-5 subunit alpha; laminin-6 subunit alpha; laminin-7 subunit alpha; epiligrin 170 kda subunit; epiligrin alpha 3 subunit; laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin); laminin, alpha 3; Epiligrin 170 kDa subunit; E170; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha

LAMA3

LAMA3;LAMA3;E170;LOCS;BM600;LAMNA;lama3a;LAMNA;E170

LAMA3_HUMAN

人类

1724

This assay has high sensitivity and excellent specificity for detection of Human LAMA3. No significant cross-reactivity or interference between Human LAMA3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 9.4 ng/ml-600 ng/ml. Sensitivity: 2.35 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LAMA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAMA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

38045908

NP_000218.2

NM_000227.3

Q16787

366,649 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Anchoring Fibril Formation Pathway (730307); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Collagen Formation Pathway (645288); Degradation Of The Extracellular Matrix Pathway (576263); ECM Proteoglycans Pathway (833812)

Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. The protein encoded by this gene is the alpha-3 subunit of laminin 5, which is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

LAMA3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS). LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 18q11.2. Cellular Component: laminin-5 complex; laminin-1 complex; extracellular region; basement membrane. Molecular Function: structural molecule activity; receptor binding. Biological Process: regulation of cell adhesion; extracellular matrix disassembly; extracellular matrix organization and biogenesis; hemidesmosome assembly; epidermis development; regulation of embryonic development; cell adhesion; regulation of cell migration. Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Laryngoonychocutaneous Syndrome; Epidermolysis Bullosa, Junctional, Herlitz Type

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800