ELISA/assay

Rat Peptidyl-glycine alpha-amidating monooxygenase, PAM ELISA Kit

MBS9325946

48-Strip-Wells

470美元

酶联免疫吸附试剂盒

Rat Peptidyl-glycine alpha-amidating monooxygenase, PAM ELISA Kit

[peptidylglycine alpha-amidating monooxygenase]

[Rat Peptidyl-glycine alpha-amidating monooxygenase (PAM) ELISA kit; PAL; PHM; pancreatic peptidylglycine alpha-amidating monooxygenase; peptidyl alpha-amidating enzyme; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; peptidylglycine 2-hydroxylase; peptidylglycine a; peptidylglycine alpha-amidating monooxygenase]

[peptidyl-glycine alpha-amidating monooxygenase; Peptidyl-glycine alpha-amidating monooxygenase; peptidyl-glycine alpha-amidating monooxygenase; peptidylglycine alpha-amidating monooxygenase; Peptidylamidoglycolate lyase; PAL]

[PAM]

[Pam; Pam; PAM; PHM; PAL]

AMD_RAT

大鼠

Store all reagents at 2-8 degree C

Samples: Plant Body Fluids And Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 1.56U/L-50U/L. Sensitivity: 1.0U/L.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determine the level of PAL (hereafter termed "analyte") in undiluted original Plant body fluids and tissue homogenates. This kit is NOT suitable for assaying non-biological sources of substances.

19923674

NP_037132.2

NM_013000.2

P14925

108,675 Da

catalyzes conversion of glycine-extended peptides into biologically active alpha-amidated peptides [RGD, Feb 2006]

PAM: Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. 6 isoforms of the human protein are produced by alternative splicing. Protein type: EC 1.14.17.3; Membrane protein, integral; Lyase; Vesicle; Oxidoreductase; EC 4.3.2.5. Cellular Component: extracellular space; neuron projection; cell surface; cell soma; membrane; perinuclear region of cytoplasm; secretory granule membrane; integral to membrane; plasma membrane; perikaryon; trans-Golgi network; secretory granule. Molecular Function: protein binding; copper ion binding; zinc ion binding; L-ascorbic acid binding; peptidylamidoglycolate lyase activity; calcium ion binding; protein kinase binding; peptidylglycine monooxygenase activity. Biological Process: limb development; response to drug; lactation; maternal process involved in pregnancy; central nervous system development; heart development; response to glucocorticoid stimulus; long-chain fatty acid metabolic process; response to estradiol stimulus; regulation of transcription from RNA polymerase II promoter; ovulation cycle process; odontogenesis; regulation of protein secretion; peptide metabolic process; response to copper ion; peptide amidation; regulation of actin cytoskeleton organization and biogenesis; toxin metabolic process; protein metabolic process; response to hypoxia; protein homooligomerization; protein amino acid amidation; response to pH

48-Strip-Wells

470美元

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095