抗体

磷酸化PI3 p85-甲/丙(Tyr467/199)抗体

MBS9382339

0.1毫升

440美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫细胞化学

抗体

磷酸化PI3 p85-甲/丙(Tyr467/199)抗体

[磷脂酰肌醇(-3)激酶p85-甲/丙]

[GRB1, P85A, PI3-kinase p85-alpha subunit, PI3K, PI3K p85-alpha, Phosphatidylinositol 3-kinase regulatory alpha subunit, PtdIns-3-kinase p85-alpha; P55G, PI3-kinase p85-gamma subunit, PI3K p85-gamma, Phosphatidylinositol 3-kinase regulatory gamma subunit, PtdIns-3-kinase p85-gamma, p55PIK]

[phosphatidylinositol 3-kinase regulatory subunit alpha isoform 4; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit 1; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha]

[PIK3R1; PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA; GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha]

多克隆

IgG

兔

人类, 小鼠, 大鼠

361

Phospho-PI3-kinase p85- alpha/ gamma (Tyr467/199) Antibody detects endogenous levels of PI3-kinase p85- alpha/ gamma only when phosphorylated at Tyrosine 467/199

免疫原亲和纯化

Store at -20 °C. Stable for 12 months from date of receipt

免疫印迹(免疫印迹), 免疫荧光(IF), 免疫细胞化学(免疫细胞化学)

WB 1:500-1:2000. IF/ICC 1:100-1:500. Optimal dilutions/concentrations should be determined by the end user.

免疫印迹

Immunogen: A synthesized peptide derived from human PI3-kinase p85- alpha/ gamma around the phosphorylation site of Tyrosine 467/199.

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

PIK3R1 is a regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids.

335057534

NP_001229395.1

NM_001242466.1

P27986

54, 83kDa

3-phosphoinositide Biosynthesis Pathway (545314); 3-phosphoinositide Biosynthesis Pathway (138600); AMPK Signaling Pathway (198868); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807); Amoebiasis Pathway (167324)

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

0.1毫升

440美元

0.2毫升

715