图像 1 :
Immunohistochemistry analysis of paraffin-embedded human brain tissue using VE-Cadherin (Phospho-Tyr731) antibody.
图像 2 :
Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3mM, 40mins), using VE-Cadherin (Phospho-Tyr731) antibody.
[7B4 antigen; CADH5; Cadherin-5; CD144 antigen; Vascular endothelial-cadherin precursor; VE-cadherin]
[cadherin-5 preproprotein; Cadherin-5; cadherin-5; cadherin 5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144]
Human (Identities = 100%, Positives = 100%);. Mouse (Identities = 92%, Positives = 100%)
VE-Cadherin (Phospho-Tyr731) antibody detects endogenous levels of VE-Cadherin only when phosphorylated at tyrosine 731.
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Store at -20 degree C/1 year
WB: 1:500~1:3000。 IHC: 1:50~1:100。 ELISA: 1:20000
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human VE-Cadherin around the phosphorylation site of tyrosine 731 (H-I-YP-G-Y). All Sites: Human: Tyr731; Mouse Tyr731
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
NCBI GI登录号 :
Adherens Junctions Interactions Pathway (119533); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); S1P2 Pathway (138059); Signaling Events Mediated By VEGFR1 And VEGFR2 Pathway (137940)
This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]
CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain. Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 16q21. Cellular Component: cell junction; cell surface; intercellular junction; membrane; plasma membrane. Molecular Function: beta-catenin binding; protein binding; receptor binding. Biological Process: intercellular junction assembly; transforming growth factor beta receptor signaling pathway
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308