抗体

DYRK1A/B(磷酸化Tyr321/273)抗体

MBS9384609

0.1毫升

440美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化

抗体

DYRK1A/B(磷酸化Tyr321/273)抗体

[DYRK1A/B]

[dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 1; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; dual specificity tyrosine-phosphorylation-regulated kinase 1A; dual specificity tyrosine phosphorylation regulated kinase 1A; Dual specificity YAK1-related kinase; HP86; Protein kinase minibrain homolog; MNBH; hMNB]

[DYRK1A/B]

[DYRK1A;DYRK1A;MNB;DYRK;HP86;MNBH;MRD7;DYRK1;DYRK;MNB;MNBH;MNBH;hMNB]

多克隆

IgG

兔

人类, 小鼠, 大鼠

DYRK1A/B (Phospho-Tyr321/273) Ab detects endogenous levels of DYRK1A/B only when phosphorylated at Tyr321/273.

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油

1毫克/毫升

Store at -20°C. Stable for 12 months from date of receipt.

免疫印迹(免疫印迹), 免疫组化(免疫组化)

WB 1:1000-3000, IHC 1:50-1:200. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.

免疫印迹(免疫印迹)

免疫组化(免疫组化)

免疫组化(免疫组化)

免疫组化(免疫组化)

At 1/200 staining Human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.

Immunogen: A synthesized peptide derived from human DYRK1A/B around the phosphorylation site of Tyr321/273. Subcellular Location: Nucleus speckle.

Tissue Specificity: Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney. Similarity: The polyhistidine repeats act as targeting signals to nuclear speckles.Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

18765758

NP_001387.2

NM_001396.4

Q13627

70KD

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); G0 And Early G1 Pathway (477139); Hedgehog Signaling Pathway (198835); Mitotic G1-G1/S Phases Pathway (160941)

This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq, Jul 2008]

DYRK1A: a dual-specificity protein kinase of the DYRK family. Contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. May play a significant role in a pathway regulating cell proliferation and may be involved in brain development. Its gene is localized in the Down syndrome (DS) critical region of chromosome 21, and is a strong candidate gene for learning defects associated with Down syndrome. Overexpression in mice gives rise to neurological abnormalities similar to those observed in DS. Five splice-variant isoforms have been described. Protein type: CMGC group; DYRK family; Dyrk1 subfamily; EC 2.7.12.2; Kinase, protein; Protein kinase, CMGC; Protein kinase, dual-specificity (non-receptor). Chromosomal Location of Human Ortholog: 21q22.13. Cellular Component: nuclear speck; nucleoplasm; nucleus. Molecular Function: non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein self-association; protein serine/threonine kinase activity; protein-tyrosine kinase activity; tau protein binding. Biological Process: circadian rhythm; negative regulation of DNA damage response, signal transduction by p53 class mediator; nervous system development; peptidyl-threonine phosphorylation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation. Disease: Mental Retardation, Autosomal Dominant 7

0.1毫升

440美元

0.2毫升

715