产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
组蛋白H3小鼠单克隆抗体
目录 :
MBS9400490
规格 :
0.05毫升
价格 :
200美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS9400490 图像 1
Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, using MBS9400490 diluted at 1:5,000.
图像 2 :
MyBioSource MBS9400490 图像 2
IF analysis of Hela with DAPI (Left) and MBS9400490(Right) diluted at 1:200.
图像 3 :
MyBioSource MBS9400490 图像 3
Input: Hela Cell Lysate. 2B'B'IP product: IP dilute 1:200. Western blot analysis: primary antibody MBS9400490: 1:2,000. Secondary antibody: Goat anti-Mouse IgG(H+L) 1:10,000.
产品信息
目录号 :
MBS9400490
产品类型 :
抗体
产品全称 :
组蛋白H3小鼠单克隆抗体
产品简称 :
[组蛋白H3]
产品名称同义词 :
[H3/A;H3FA;H3FB;H3FC]
其他名称 :
[histone H3.1; Histone H3.1; histone H3.1; histone cluster 1, H3a; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l]
其他基因名称 :
[HIST1H3A;HIST1H3A;H3/A;H3FA;H3FA]
UniProt数据库进入名 :
H31_HUMAN
克隆性 :
单克隆
宿主 :
小鼠
反应物种 :
人类, 大鼠, 小鼠
序列长度 :
136
特异性 :
The Histone H3 antibody can detect endogenous Histone H3 protein.
纯度 :
亲和纯化美国免疫原
形式 :
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
浓度 :
1.0毫克/毫升
储存稳定性 :
Store at -20 degree C
检测过的应用 :
免疫印迹(免疫印迹), 免疫荧光(IF), 免疫沉淀(免疫沉淀)
应用笔记 :
Western blotting: 1:2000~1:5000. Immunofluorescence: 1:100-500. Immunoprecipitation: 1:200
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫荧光(IF)
image3头 :
试验数据
其他信息2 :
Target Name: Histone H3
产品种类 :
总数蛋白Ab
产品描述 :
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
NCBI GI登录号 :
4504281
NCBI登录号 :
NP_003520.1
NCBI基因登录号 :
NM_003529.2
UniProt数据库登录号 :
P68431
NCBI分子量 :
17 kd
NCBI信号通路 :
Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway (1269513); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amyloids Pathway (1269169); Cell Cycle Pathway (1269741); Cell Cycle, Mitotic Pathway (1269763); Cellular Senescence Pathway (1270426); Cellular Responses To Stress Pathway (1270414); Chromatin Modifying Enzymes Pathway (1270434); Chromatin Organization Pathway (1270433)
NCBI总结 :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt数据库总结 :
H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 6p22.2. Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus. Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity. Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; regulation of gene expression, epigenetic; DNA methylation on cytosine; DNA replication-dependent nucleosome assembly
尺寸1 :
0.05毫升
价格1 :
200美元
尺寸2 :
0.1毫升
价格2 :
255
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。