人类, 小鼠, 大鼠
图像 1 :
Western blot analysis of extracts of Jurkat cell lines, using PTPN22 antibody.
[tyrosine-protein phosphatase non-receptor type 22 isoform 3; Tyrosine-protein phosphatase non-receptor type 22; tyrosine-protein phosphatase non-receptor type 22; protein tyrosine phosphatase, non-receptor type 22 (lymphoid); Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; LyP; PEST-domain phosphatase; PEP]
[PTPN22; PTPN22; LYP; PEP; LYP1; LYP2; PTPN8; PTPN8; LyP; PEP]
人类, 小鼠, 大鼠
Antibodies were purified by affinity purification using immunogen.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Store at -20 degree C
Western blotting: 1:500 - 1:2000
Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human PTPN22.
Target Name: PTPN22
PTPN22 (Lyp/PEP) is a cytoplasmic phosphatase expressed by hematopoietic cells (1,2). PTPN22 associates with the tyrosine kinase Csk to inhibit T cell receptor signaling through inactivation of Src kinases (3,4). Csk phosphorylates Src kinases on an inhibitory tyrosine, while PTPN22 dephosphorylates an activating site (4). PTPN22(-/-) mice have higher levels of activated Lck than wild-type, resulting in greater T cell expansion and increased serum antibody levels (5). Research studies have shown that a single-nucleotide polymorphism, 1858T of the PTPN22 gene which encodes the amino acid substitution R620W, confers increased risk for multiple autoimmune diseases including type I diabetes, rheumatoid arthritis, systemic lupus erythematosus, and Graves disease (6-9). Interestingly, although the R620W substitution disrupts the interaction between Csk and PTPN22, it is actually a gain-of-function mutation resulting in increased phosphatase activity (6,10,11). Recent evidence suggests that the autoimmune phenotype associated with the R620W variant is the result of increased calpain-mediated degradation and decreased protein levels of PTPN22 (12).
NCBI GI登录号 :
This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009]
PTPN22: Seems to act on Cbl. May play a role in regulating the function of Cbl and its associated protein kinases. Acts as negative regulator of T-cell receptor (TCR) signaling. Dephosphorylates and inactivates the SRC family kinases. Interacts with CSK. Interacts with LPXN. Predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells. Down-regulated by phosphorylation. Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Protein phosphatase, tyrosine (non-receptor); EC 184.108.40.206; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1p13.2. Cellular Component: internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasm; nucleus. Molecular Function: protein binding; protein tyrosine phosphatase activity; kinase binding; SH3 domain binding. Biological Process: regulation of B cell receptor signaling pathway; regulation of natural killer cell proliferation; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation; negative regulation of T cell activation; regulation of innate immune response; T cell differentiation. Disease: Diabetes Mellitus, Insulin-dependent; Systemic Lupus Erythematosus; Rheumatoid Arthritis
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