产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Wilms瘤抑癌基因抗体
目录 :
MBS9410783
规格 :
0.1毫升
价格 :
255美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS9410783 图像 1
Western blot analysis of kidney cell lysate using WT1 antibody.
图像 2 :
MyBioSource MBS9410783 图像 2
Immunohistochemical analysis of paraffin-embedded human kidney cancer using WT1 antibody at dilution of 1:500 (400x lens).
图像 3 :
MyBioSource MBS9410783 图像 3
Immunohistochemical analysis of paraffin-embedded rat lung using WT1 antibody at dilution of 1:500 (400x lens).
产品信息
目录号 :
MBS9410783
产品类型 :
抗体
产品全称 :
Wilms瘤抑癌基因抗体
产品简称 :
[Wilms瘤抑癌基因]
产品名称同义词 :
[GUD;AWT1;WAGR;WT33;NPHS4]
其他名称 :
[肾母细胞瘤蛋白质亚型A;肾母细胞瘤蛋白;肾母细胞瘤蛋白;肾母细胞瘤1;WT33]
产品基因名称 :
[Wilms瘤抑癌基因]
其他基因名称 :
[Wilms瘤抑癌基因;Wilms瘤抑癌基因;GUD;AWT1;WAGR;WT33;NPHS4;机智-2;EWS-Wilms瘤抑癌基因]
UniProt数据库进入名 :
WT1_HUMAN
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
序列长度 :
497
特异性 :
抗体检测内生水平总数WT基因蛋白
纯度 :
Antibodies were purified by affinity purification using immunogen.
形式 :
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
浓度 :
1.0毫克/毫升
储存稳定性 :
存放在-20°C
检测过的应用 :
免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫荧光(IF)
应用笔记 :
Western blotting: 1:500 - 1:2000. Immunohistochemistry: 1:50 - 1:100. Immunofluorescence: 1:50 - 1:200
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
image3头 :
免疫组化(免疫组化)
image4头 :
免疫荧光(IF)
image4描述 :
Immunofluorescence analysis of HeLa cell using WT1 antibody. Blue: DAPI for nuclear staining.
其它信息1 :
Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human WT1. SDS-PAGE MW: 49kD
其他信息2 :
Target Name: WT1
产品种类 :
总数蛋白Ab
产品描述 :
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
NCBI GI登录号 :
65507714
NCBI登录号 :
NP_000369.3
NCBI基因登录号 :
NM_000378.4
UniProt数据库登录号 :
P19544
NCBI信号通路 :
Integrated Pancreatic Cancer Pathway (711360); Regulation Of Telomerase Pathway (137987); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)
NCBI总结 :
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
UniProt数据库总结 :
Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
尺寸1 :
0.1毫升
价格1 :
255美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。