产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
MMP1抗体
目录 :
MBS9602703
规格 :
0.05毫升
价格 :
235美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 流式细胞仪, 酶免疫法
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS9602703 图像 1
Figure 1: Western blot analysis using MMP1 mAb against HEK293 (1) and MMP1(AA: 24-213)-hIgGFc transfected HEK293 (2) cell lysate.
图像 2 :
MyBioSource MBS9602703 图像 2
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) using NFKB1 mouse mAb with DAB staining.
图像 3 :
MyBioSource MBS9602703 图像 3
Immunofluorescence analysis of Hela cells using MMP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. / Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
产品信息
目录号 :
MBS9602703
产品类型 :
抗体
产品全称 :
MMP1抗体
产品简称 :
[MMP1]
其他名称 :
[interstitial collagenase isoform 1 preproprotein; Interstitial collagenase; interstitial collagenase; matrix metallopeptidase 1; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1]
产品基因名称 :
[MMP1]
其他基因名称 :
[MMP1;MMP1;CLG;CLGN;CLG;基质金属蛋白酶-1]
克隆性 :
单克隆
抗体亚型 :
IgG1
宿主 :
小鼠
反应物种 :
人类
序列长度 :
469
特异性 :
MMP1抗体检测内生水平总数MMP1
纯度 :
亲和色谱
形式 :
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
浓度 :
1毫克/毫升
储存稳定性 :
Store at -20°C. Stable for 12 months from date of receipt.
检测过的应用 :
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
应用笔记 :
ELISA: 1:10000. WB: 1:500-1:2000. IHC: 1:200-1:1000. ICC: 1:200-1:1000. FC/FACS: 1:200-1:400. IMPORTANT: For western blots, incubate membrane with diluted Ab in 5% w/v milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
image1头 :
免疫印迹(免疫印迹)
image2头 :
免疫组化(免疫组化)
image3头 :
免疫荧光(IF)
其它信息1 :
Immunogen: Purified recombinant fragment of human MMP1 expressed in E. Coli.
产品描述 :
Description: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Belongs to the peptidase M10A family.
NCBI GI登录号 :
4505215
NCBI登录号 :
NP_002412.1
NCBI基因登录号 :
NM_002421.3
UniProt数据库登录号 :
P03956
NCBI分子量 :
54 kDa
NCBI信号通路 :
Activation Of Matrix Metalloproteinases Pathway (576264); Basigin Interactions Pathway (106065); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cell Surface Interactions At The Vascular Wall Pathway (106062); Degradation Of Collagen Pathway (730309); Degradation Of Collagen Pathway (762258); Degradation Of The Extracellular Matrix Pathway (576263); Degradation Of The Extracellular Matrix Pathway (762256); Endothelins Pathway (137958)
NCBI总结 :
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
UniProt数据库总结 :
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:2557822, PubMed:2153297, PubMed:1645757). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369).
尺寸1 :
0.05毫升
价格1 :
235美元
尺寸2 :
0.1毫升
价格2 :
295
size3 :
0.2毫升
价格3 :
370
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。