抗体

TOP3B抗体

MBS9604527

0.1毫升

240美元

多克隆

兔

未共轭

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 酶免疫法

抗体

TOP3B抗体

[TOP3B]

[DNA topoisomerase 3 beta 1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta 1; DNA topoisomerase III beta-1; FLJ39376; TOP 3B; TOP3B; TOP3B_HUMAN; TOP3B1; Topo III beta; Topoisomerase (DNA) III beta; Topoisomerase DNA III beta; Topoisomerase III beta]

[DNA topoisomerase 3-beta-1 isoform 1; DNA topoisomerase 3-beta-1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta; DNA topoisomerase III beta-1]

[TOP3B]

[TOP3B;TOP3B;TOP3B1;TOP3B1]

多克隆

IgG

兔

人类, 小鼠

TOP3B抗体检测内生水平总数TOP3B

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油

1毫克/毫升

Store at -20 degree C. Stable for 12 months from date of receipt.

免疫印迹(免疫印迹), 免疫荧光(IF), 免疫细胞化学(免疫细胞化学), 酶联免疫吸附测定(EIA)

WB: 1:500-1:1000. IF/ICC: 1:100-1:500. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.

免疫荧光(IF)

免疫印迹(免疫印迹)

Immunogen: A synthesized peptide derived from human TOP3B, corresponding to a region within C-terminal amino acids.

Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity. Similarity: Belongs to the type IA topoisomerase family.

530788288

NP_001269041.1

NM_001282112.1

O95985

96 KD

Bloom's Syndrome Complex Pathway (413477); Bloom's Syndrome Complex Pathway (890629); Fanconi Anemia Pathway (377262); Fanconi Anemia Pathway (377128); Homologous Recombination Pathway (83046); Homologous Recombination Pathway (454)

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (). Possesses negatively supercoiled DNA relaxing activity.

0.1毫升

240美元

0.2毫升

280