抗体

MLH1抗体

MBS9605077

0.1毫升

240美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 酶免疫法

抗体

MLH1抗体

[MLH1]

[DNA mismatch repair protein Mlh1 isoform 1; DNA mismatch repair protein Mlh1; DNA mismatch repair protein Mlh1; mutL homolog 1; MutL protein homolog 1]

[MLH1]

[MLH1;MLH1;FCC2;COCA2;HNPCC;hMLH1;HNPCC2;COCA2]

多克隆

IgG

兔

人类, 小鼠, 大鼠

756

MLH1抗体检测内生水平总数MLH1

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油

1毫克/毫升

Store at -20 degree C. Stable for 12 months from date of receipt.

免疫印迹(免疫印迹), 免疫荧光(IF), 免疫细胞化学(免疫细胞化学), 酶联免疫吸附测定(EIA)

WB: 1:500-1:1000. IF/ICC: 1:100-1:500. IMPORTANT: For western blots, incubate membrane with diluted Ab in 5% w/v milk, 1 x TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.

免疫印迹(免疫印迹)

Immunofluorescene (IF)

免疫印迹(免疫印迹)

Immunogen: A synthesized peptide derived from human MLH1, corresponding to a region within the internal amino acids.

Function: Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. Similarity: Belongs to the DNA mismatch repair MutL/HexB family.

4557757

NP_000240.1

NM_000249.3

P40692

85 KD

BRCA1-associated Genome Surveillance Complex (BASC) Pathway (413428); Colorectal Cancer Pathway (83106); Colorectal Cancer Pathway (518); Direct P53 Effectors Pathway (137939); Endometrial Cancer Pathway (83109); Endometrial Cancer Pathway (521); Fanconi Anemia Pathway (377262); Fanconi Anemia Pathway (377128); Meiosis Pathway (477133); Meiotic Recombination Pathway (205242)

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

0.1毫升

240美元

0.2毫升

280