人类, 小鼠, 大鼠
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 酶免疫法
图像 1 :
Western blot analysis of extracts from rat brain, using PSAP Antibody. Lane 1 was treated with the blocking peptide.
图像 2 :
MBS9605785 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
图像 3 :
Western blot analysis of extracts from NIH/3T3 cells using PSAP antibody.The lane on the left is treated with the antigen-specific peptide.
[A1 activator; Cerebroside sulfate activator; Co-beta-glucosidase; Component C; CSAct; Dispersin; GLBA; Glucosylceramidase activator; Proactivator polypeptide; Proactivator polypeptide precursor; Prosaposin (sphingolipid activator protein 1); prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy); Prosaposin; Protein A; Protein C; PSAP; SAP-1; SAP-2; SAP_HUMAN; SAP1; Saposin A; Saposin B; Saposin B Val; Saposin C; Saposin D; Saposin-D; Saposins; Sgp1; Sphingolipid activator protein 1; Sphingolipid activator protein 2; Sulfated glycoprotein 1; Sulfatide/GM1 activator]
[prosaposin isoform b preproprotein; Prosaposin; prosaposin; prosaposin; Proactivator polypeptide]
人类, 小鼠, 大鼠
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
液体。 磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
Store at -20 degree C. Stable for 12 months from date of receipt.
免疫印迹(免疫印迹), 免疫荧光(IF), 免疫细胞化学(免疫细胞化学), 酶联免疫吸附测定(EIA)
WB: 1:500-1:1000。 IF/ICC: 1:100-1:500
Immunogen: A synthesized peptide. Subcellular Location: Lysosome. Predicted Cross Reactivity : Bovine, Sheep, Rabbit, Dog . Similarity: Bovine (83%), Sheep (83%), Rabbit (91%), Dog (83%)
Function: Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 22.214.171.124) and galactosylceramide by beta-galactosylceramidase (EC 126.96.36.199). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. Subunit Structure: Saposin-B is a homodimer. Prosaposin exists as a roughly half-half mixture of monomers and disulfide-linked dimers (PubMed:10406958, PubMed:12510003, PubMed:7730378, PubMed:21835174). Monomeric prosaposin interacts (via C-terminus) with sortilin/SORT1, the interaction is required for targeting to lysosomes (PubMed:14657016, PubMed:22431521). Post-translational Modifications: The lysosomal precursor is proteolytically processed to 4 small peptides, which are similar to each other and are sphingolipid hydrolase activator proteins. N-linked glycans show a high degree of microheterogeneity. The one residue extended Saposin-B-Val is only found in 5% of the chains.
NCBI GI登录号 :
Glycosphingolipid Metabolism Pathway (530751); Hemostasis Pathway (106028); Lysosome Pathway (99052); Lysosome Pathway (96865); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Sphingolipid Metabolism Pathway (119543)
This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 188.8.131.52) and galactosylceramide by beta-galactosylceramidase (EC 184.108.40.206). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 220.127.116.11), GM1 gangliosides by beta-galactosidase (EC 18.104.22.168) and globotriaosylceramide by alpha-galactosidase A (EC 22.214.171.124). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 126.96.36.199).Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.
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