产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类钙调蛋白2/CALM2抗体
目录 :
ACA0830
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
J4D8
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类钙调蛋白2/CALM2抗体
目录号(SKU号) :
ACA0830
NCBI :
NP_001734
描述 :
Calmodulin has been known to act as an intracellular calcium sensor protein. When the intracellular Ca2+ concentration increases, calmodulin can bind up to four Ca2+, changing its conformation and regulating cellular functions such as activation or inhibition of a large number of enzymes, ion channels, and receptors.
别称 :
PHKD, CAMII, PHKD2, Calmodulin 2, CALM 2, Calmodulin2, CAM II, LP7057 protein, PHKD 2, Phosphorylase kinase delta, Calmodulin-2, CALM2
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Zhang M., et al. (1998). Biochem Cell Biol. 76(2-3):313-23.;; James.P., et al. (1995). Trend Biochem Sci. 20: 38-42.
ab宿主 :
小鼠
抗体亚型 :
IgG2a kappa
免疫原 :
重组人类钙调蛋白(1-149aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪
IF Comment And Pic :
ICC/IF analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calmodulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: NIH3T3 cell lysate ;;Lane 2.: HeLa cell lysate ;;Lane 3.: Jurkat cell lysate ;;Lane 4.: A549 cell lysate ;;Lane 5.: MCF7 cell lysate ;;Lane 6.: Ramos cell lysate ;;Lane 7.: PC3 cell lysate
反应对象 :
人类
克隆编号 :
J4D8
Ab用途 :
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
FACS Comment and Pic :
Flow cytometry analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
抗原物种 :
人类
基因类别 :
信号转导
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-G affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
P0DP24
内部搜索条款 :
钙调蛋白, ACA0830-10微克, ACA0830-20微克, ACA0830-50微克, ACA0830-100微克, ACA0830-250微克, ACA0830-500微克, ACA0830-1毫克, ACA0830-10, ACA0830-20, ACA0830-50, ACA0830-100, ACA0830-250, ACA0830-500, ACA0830-1
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司，制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。