抗体

人类CD44抗体

ACD0826

50微升

170美元

单克隆

小鼠

未共轭

5c10

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

人类CD44抗体

ACD0826

NP_001001390

CD44 is a broadly distributed transmembrane glycoprotein that plays a critical role in a variety of cellular behaviors, including adhesion, migration, invasion, and survival. CD44 mediates cell-cell and cell-matrix interactions in a large part through its affinity for hyaluronan (HA), a glycosaminoglycan constituent of extracellular matrices, but also potentially through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Variants of CD44 are expressed in tissues during development, including embryonic epithelia. known functions of CD44 are cellular adhesion (aggregation and migration), hyaluronate degradation, lymphocyte activation, lymph node homing, myelopoiesis and lymphopoiesis, angiogenesis, and release of cytokines. The functions of CD44 are principally dependant on cellular adhesion in one setting or another.

CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, LHR, MDU2, MDU3, MIC4

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Cichy, J. and Pure, E. (2003) J.Cell Biol. 161, 839-843.;;Sneath RJ, Mangham DC (1998) Mol Pathol. 51(4):191-200.

小鼠

IgG2b kappa

重组人类CD44(21-145aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪

ICC/IF analysis of CD44 in HeLa cells. The cell was stained with ACD0826 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The recombinant protein (200ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD44 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human CD44 protein

人类

5C10

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS nalysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of CD44 in HeLa cells. The cell was stained with ACD0826 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). Flow cytometry analysis of CD44 in PBMC cells. The cell was stained with ACD0826 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). {ACD0826-FC2.jpg}

人类

免疫学

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P16070

CD44, ACD0826-10微克, ACD0826-20微克, ACD0826-50微克, ACD0826-100微克, ACD0826-250微克, ACD0826-500微克, ACD0826-1毫克, ACD0826-10, ACD0826-20, ACD0826-50, ACD0826-100, ACD0826-250, ACD0826-500, ACD0826-1