抗体

人类CIB1抗体

ACI0807

50微升

170美元

单克隆

小鼠

未共轭

1D1

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

人类CIB1抗体

ACI0807

NP_006375

CIB1 (Calcium and integrin binding 1) is regulatory protein with 50% homology to calmodulin and calcineurin B, that encodes a member of the calcium-binding protein family. The specific function of this protein has not yet been determined; however this protein is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining. Also CIB1 is widely expressed and binds to a number of effectors, such as integrin alphaIIb, PAk1, and polo-like kinases in different tissues.

Calcium and integrin binding 1, CIB, KIP (Kinase interacting protein), SIP2-28(SNK interacting protein2-28), CALMYRIN, CIB1, Calcium and integrin binding 1 Calcium and integrin binding 1 (calmyrin), Calcium and integrin binding protein 1, CIB, DNA PKcs interacting protein, Human Snk interacting protein 2 to 28 mRNA complete cds, Kinase interacting protein, PRKDCIP, SIP2 28, SNK interacting protein 2 to 28

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Yuan W, et al., (2006) Mol Cell Biol 26(22): 8507-14.;;Yuan W, et al., (2006) J Cell Biol 16;; 172(2):169-75.

小鼠

IgG2b kappa

重组人类CIB1(1-191aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

ICC/IF analysis of CIB1 in HeLa cells. The cell was stained with ACI0807 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysate of HT29 (40ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CIB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CIB1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: CIB1 Transfected 293T cell lysate {ACI0807-WB2.jpg}

人类

1D1

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

人类

信号转导

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

Q99828

CIB1, ACI0807-10微克, ACI0807-20微克, ACI0807-50微克, ACI0807-100微克, ACI0807-250微克, ACI0807-500微克, ACI0807-1毫克, ACI0807-10, ACI0807-20, ACI0807-50, ACI0807-100, ACI0807-250, ACI0807-500, ACI0807-1