抗体

人类HMG-1/高迁移率族蛋白B1抗体

AHM0915

50微升

170美元

单克隆

小鼠

未共轭

J2E1

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片, 其他

人类HMG-1/高迁移率族蛋白B1抗体

AHM0915

NP_002119

High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.

Sulfoglucuronyl carbohydrate binding protein, SBP-1, HMGB1, HMG3, HMG1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Palumbo R J, et al., (2004) Cell Biol. Feb 2 164(3).;; Andersson U, et al., (2002) Leukoc Biol. Dec. 72(6):1084-91.

小鼠

IgG2b kappa

重组人类高迁移率族蛋白B1(1-215aa)纯化来自杆状病毒

酶联免疫吸附测定, 免疫印迹, 免疫组化

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMGB1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;; Lane 1.: K562 cell lysate;;Lane 1.: MCF7 cell lysate;;

人类

J2E1

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

人类

Epigenomics (Transcription & Translation)

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P09429

Sulfoglucuronyl carbohydrate binding protein, SBP-1, SBP1, SBP 1, HMGB1, HMGB 1, HMG3, HMG1, HMG 1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin, HMG 3, High mobility group box1, High mobility group (nonhistone chromosomal) protein 1, High mobility group protein 1, AHM0915-10ug, AHM0915-20ug, AHM0915-50ug, AHM0915-100ug, AHM0915-250ug, AHM0915-500ug, AHM0915-1mg, AHM0915-010, AHM0915-020, AHM0915-050, AHM0915-100, AHM0915-250, AHM0915-500, AHM0915-01M

Hashizume H, et al. Skin recruitment of monomyeloid precursors involves human herpesvirus-6 reactivation in drug allergy. (Allergy. 2013){https://pubmed.ncbi.nlm.nih.gov/23573902/};;Ito H, et al. HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice. 9EMBO Mol Med. 2015){https://pubmed.ncbi.nlm.nih.gov/25510912/}