产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
Human Nanog antibody
目录 :
ANA0837
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
5A10
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
Human Nanog antibody
目录号(SKU号) :
ANA0837
NCBI :
NP_079141
描述 :
Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
别称 :
Nanog homeobox, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Tay Y., et al. (2008) Nature. 455(7216):1124-8;; Chiou SH., et al. (2008) Clin Cancer Res. 14(13):4085-95;; Boer B., et al. (2008) Mol Reprod Dev.
ab宿主 :
小鼠
抗体亚型 :
IgG2a kappa
免疫原 :
Recombinant human Nanog (1-154aa) purified from E. coli
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫组化, 流式细胞仪
WB Comment and Pic :
The cell lysate of NIH3T3 (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
反应对象 :
人类
克隆编号 :
5A10
Ab用途 :
The antibody has been tested by ELISA, Western blot, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
FACS Comment and Pic :
Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
IHC Comment And Pic :
Paraffin embedded sections of human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
抗原物种 :
人类
基因类别 :
免疫学
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-G affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
Q9H9S0
内部搜索条款 :
Nanog homeobox, Nanog, Homeobox transcription factor Nanog-delta 48, Homeobox transcription factor Nanog, Homeobox protein NANOG, hNanog, FLJ40451, FLJ12581, , , ANA0837-10ug, ANA0837-20ug, ANA0837-50ug, ANA0837-100ug, ANA0837-250ug, ANA0837-500ug, ANA0837-1mg, ANA0837-010, ANA0837-020, ANA0837-050, ANA0837-100, ANA0837-250, ANA0837-500, ANA0837-01M
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司,制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。