抗体

人类RAB5A抗体

ARA0803

50微升

170美元

单克隆

小鼠

未共轭

3A4

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

人类RAB5A抗体

ARA0803

NP_004153

Rab5a is a member of the RAS oncogene family. Rab5a is a small GTPase localized to the plasma membrane and early endosomes. It is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.

RAB5A member RAS oncogene family, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bucci C, et al., (1992) Cell 70 (5): 715-28.;;Seachrist JL, et al., (2002) J. Biol. Chem. 277 (1): 679-85.

小鼠

IgG2b kappa

重组人类Rab5a(1-215aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

ICC/IF analysis of Rab5a in U87MG cells. The cell was stained with ARA0803 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The tissue lysate of mouse brain (45ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

人类

3A4

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

人类

Cancer

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P20339

Rab5a, ARA0803-10微克, ARA0803-20微克, ARA0803-50微克, ARA0803-100微克, ARA0803-250微克, ARA0803-500微克, ARA0803-1毫克, ARA0803-10, ARA0803-20, ARA0803-50, ARA0803-100, ARA0803-250, ARA0803-500, ARA0803-1

Del Cid-Pellitero E, et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. (Front Syst Neurosci. 2017){https://pubmed.ncbi.nlm.nih.gov/28408870/};;Winer H, et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. (Nat Commun. 2018){https://pubmed.ncbi.nlm.nih.gov/30218067/}