抗体

Human Synuclein-alpha/beta antibody

ASC0632

50微升

170美元

单克隆

小鼠

未共轭

3B6

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

Human Synuclein-alpha/beta antibody

ASC0632

NP_000336, NP_003076

alpha-synuclein (amino acids 119-140), an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. alpha-synuclein has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and more recently, to be an important regulatory component of vesicular transport in neuronal cells. Specially, the non-Abeta component of amyloid plaques is a highly amyloidogenic peptide consisting of 35 amino acids which was first identified associated with senile plaques in the Alzheimer's disease brain.

Synuclein beta, Synuclein alpha, SNCB protein, SNCB, SNCA, PD1, Parkinson disease familial 1, PARK4, PARK1, Non A4 component of amyloid precursor, Non A beta component of AD amyloid, NACP, beta synuclein, alpha synuclein, a,b-Synuclein

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Jakes R., et al. (1994) FEBS lett. 345, 27-32.;; Ueda K., et al. (1993) Proc. Natl.Acad. Sci. USA 90, 11282-11286.;; Kim J. (1997) Molecules and Cells 7, 78-83.;; Paik S. R., et al. (1997) Arch. Biochem. Biophys. 344, 325-334.

小鼠

IgG1 kappa

Recombinant human a-synuclein (119-140aa) purified from E. coli

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪

ICC/IF analysis of alpha, beta-Synuclein in LNCaP cells. The cell was stained with ACS0632 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). ICC/IF analysis of alpha, beta-Synuclein in U87MG cells. The cell was stained with ACS0632 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {ASC0632-IF2.jpg}ICC/IF analysis of alpha, beta-Synuclein in C6 cells. The cell was stained with ACS0632 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ASC0632-IF3.jpg}

The recombinant protein (20ng) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human alpha-synuclein protein;;Lane 2.: Recombinant human beta-synuclein protein;;Lane 3.: Recombinant human gamma-synuclein protein;;Lane 4.: Mouse brain Tissue lysate;;Lane 5.: Rat brain Tissue lysate;;

人类

3B6

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of alpha, beta-Synuclein in LNCaP cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). Flow cytometry analysis of alpha, beta-Synuclein in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). {ASC0632-FC2.jpg}Flow cytometry analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). {ASC0632-FC3.jpg}

人类

神经科学

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P37840, Q16143

Synuclein beta, Synuclein alpha, SNCB protein, SNCB, SNCA protein, SNCA, PD1, Parkinson disease familial 1, PARK4, PARK1, Non A4 component of amyloid precursor, Non A beta component of AD amyloid, NACP, beta synuclein, alpha synuclein, a/b-Synuclein, a,b-Synuclein, a b-Synuclein, ASC0632-10ug, ASC0632-20ug, ASC0632-50ug, ASC0632-100ug, ASC0632-250ug, ASC0632-500ug, ASC0632-1mg, ASC0632-010, ASC0632-020, ASC0632-050, ASC0632-100, ASC0632-250, ASC0632-500, ASC0632-01M