产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类IL-6抗体
目录 :
ATGA0123
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT1H6
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类IL-6抗体
目录号(SKU号) :
ATGA0123
NCBI :
NP_000591
描述 :
Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length that plays critical roles in host defense, immune response and hematopoiesis. IL-6 produced by T cells, macrophages, fibroblasts, endothelial and other cells. IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-Beta, is important for developing Th17 responses. The IL-6 receptor is a trimeric complex composed of an IL-6-specific a chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.
别称 :
Interleukin-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IFNB2, HGF, HSF
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Heinrich P.C., et al. (1998) Biochem J. 334: 297-314.;; Heinrich P.C., et al. (1998) Z Ernahrungswiss. 37: 43-49.;; Jones S.A. (2005) J Immunol. 175: 3463-3468.
ab宿主 :
小鼠
抗体亚型 :
IgG2a kappa
免疫原 :
重组IL-6(30-212aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪
IF Comment And Pic :
ICC/IF analysis of IL-6 in A549 cells. The cell was stained with ATGA0123 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of IL-6 in HeLa cells. The cell was stained with ATGA0123 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{2004010104255381.jpg}ICC/IF analysis of IL-6 in Hep3B cells. The cell was stained with ATGA0123 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{2004010104258221.jpg} {ATGA0123-IF3b.jpg}
WB Comment and Pic :
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-6 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: HepG2 cell lysate ;;Lane 3.: Raw264.7 cell lysate ;;Lane 4.: PC3 cell lysate ;;Lane 5.: THP-1 cell lysate ;;Lane 6.: A549 cell lysate ;;Lane 7.: MCF7 cell lysate ;;Lane 8.: K562 cell lysate
反应对象 :
人类
克隆编号 :
AT1H6
Ab用途 :
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
FACS Comment and Pic :
Flow cytometry analysis of IL-6 in LNCaP cells. The cell was stained with ATGA0123 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).Flow cytometry analysis of IL-6 in HeLa cells. The cell was stained with ATGA0123 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).{2004010104258138.jpg}
抗原物种 :
人类
基因类别 :
免疫学
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-G affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
P05231
内部搜索条款 :
VLAB, VLA-4 subunit bet, VLA4 subunit bet, VLA 4 subunit bet, MSK12, MSK 12, MDF2, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta1, Integrin beta 1, IL-6, IL6, IL 6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, GP IIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0123-10ug, ATGA0123-20ug, ATGA0123-50ug, ATGA0123-100ug, ATGA0123-250ug, ATGA0123-500ug, ATGA0123-1mg, ATGA0123-010, ATGA0123-020, ATGA0123-050, ATGA0123-100, ATGA0123-250, ATGA0123-500, ATGA0123-01M
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司,制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。