抗体

人类KLF4抗体

ATGA0129

50微升

170美元

单克隆

小鼠

未共轭

AT4E6

免疫印迹, 酶联免疫吸附测定, 流式细胞仪

人类KLF4抗体

ATGA0129

NP_004226.3

kLF4, which is also known as the gut-enriched kruppel-like factor (GkLF), is a zinc finger-containing transcription factor that belongs to the kruppel-like family of transcription factors. kLF4 plays important roles during the proliferation and differentiation of epithelial cells. Especially, it is found predominantly in gut and has been shown to be expressed during growth arrest. kLF4 is also involved in cell cycle control since expression of kLF4 inhibits DNA synthesis by blocking the G1/S phase of the cell cycle.

krueppel-like factor 4, EZF, GKLF

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Yan FF, et al., (2008) Med Hypotheses. 70(4):845-7.;; Cho YG, et al., (2007) APMIS. 115(7):802-8.

小鼠

IgG1 kappa

重组人类KLF4(1-170aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 流式细胞仪

The recombinant protein (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLF4 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human KLF4 protein

人类

AT4E6

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of KLF4 in A549 cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).Flow cytometry analysis of KLF4 in HeLa cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).{ATGA0129-FC2.jpg}

人类

酶&蛋白酶

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

O43474

KLF4, ATGA0129-10微克, ATGA0129-20微克, ATGA0129-50微克, ATGA0129-100微克, ATGA0129-250微克, ATGA0129-500微克, ATGA0129-1毫克, ATGA0129-10, ATGA0129-20, ATGA0129-50, ATGA0129-100, ATGA0129-250, ATGA0129-500, ATGA0129-1

Petrova A, et al. Induced Pluripotent Stem Cell Differentiation and Three-Dimensional Tissue Formation Attenuate Clonal Epigenetic Differences in Trichohyalin. (Stem Cells Dev. 2016){https://pubmed.ncbi.nlm.nih.gov/27460132/}