抗体

人类CRABP2抗体

ATGA0134

50微升

170美元

单克隆

小鼠

未共轭

AT2E11

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

人类CRABP2抗体

ATGA0134

NP_001869

The cellular retinoic acid-binding protein II (CRABP-II) is involved in the conversion of vitamin A into its intracellular active form retinoic acid, which regulate the genes responsible for lipid metabolism and adipocyte differentiation. CRABP2 gene is located on chromosome 1q21-23 and this region has been linked with related disorders such as familial combined hyperlipidemia (FCHL) and type 2 diabetes mellitus.

Cellular retinoic acid binding protein 2, RBP6, CRABP-II, Cellular retinoic acid binding protein 2, CRABP2, RBP6, CRABP-II, Cellular retinoic acid binding protein 2 Cellular retinoic acid binding protein II, CRABP II, CRABPII

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Astrom,A., et al (1991). J. Biol. Chem. 266 (26), 17662-17666.;; Gupta,A., et al (2006). Cancer Res. 66 (16), 8100-8108.

小鼠

IgG2a kappa

重组人类CRABP2(1-138aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

ICC/IF analysis of CRABP2 in HeLa cells. The cell was stained with ATGA0134 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of CRABP2 in MCF7 cells. The cell was stained with ATGA0134 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0134-IF2.jpg}

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP2 antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1. : MCF-7 cell lysate The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1. : 293T cell lysate;; Lane 2. : CRABP2 Transfected 293T cell lysate{ATGA0134-WB2.jpg}

人类

AT2E11

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

人类

Epigenomics (Transcription & Translation)

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P29373

CRABP2, ATGA0134-10微克, ATGA0134-20微克, ATGA0134-50微克, ATGA0134-100微克, ATGA0134-250微克, ATGA0134-500微克, ATGA0134-1毫克, ATGA0134-10, ATGA0134-20, ATGA0134-50, ATGA0134-100, ATGA0134-250, ATGA0134-500, ATGA0134-1