抗体

Aequorea victoria GFP antibody

ATGA0148

50微升

170美元

单克隆

小鼠

未共轭

AT2G5

免疫印迹, 酶联免疫吸附测定

Aequorea victoria GFP antibody

ATGA0148

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

绿色荧光蛋白

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Phillips G (2001) FEMS Microbiol Lett 204(1):9-18.;;Tsien R (1998) Annu Rev Biochem 67:509-544.;;Prasher, et al., (1992) Gene 111(2):229-233.

小鼠

IgG2a kappa

重组绿色荧光蛋白(1-238aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹

The recombinant proteins were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-GFP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human GFP protein (20ng);;Lane 2.: Recombinant human GFP protein (40ng);;Lane 3.: Recombinant human GFP protein (80ng)The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GFP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: GFP transfected 293T cell lysate {ATGA0148-WB2.jpg}

Aequorea victoria

AT2G5

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

绿色荧光蛋白

Fusion Tag & Detection

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P42212

绿色荧光蛋白, ATGA0148-10微克, ATGA0148-20微克, ATGA0148-50微克, ATGA0148-100微克, ATGA0148-250微克, ATGA0148-500微克, ATGA0148-1毫克, ATGA0148-10, ATGA0148-20, ATGA0148-50, ATGA0148-100, ATGA0148-250, ATGA0148-500, ATGA0148-1