抗体

人类ERK2/MAPK1抗体

ATGA0182

50微升

170美元

单克隆

小鼠

未共轭

AT1A4

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

人类ERK2/MAPK1抗体

ATGA0182

NP_620407

Mitogen-activated protein kinases (MAPKs) are reported to be critical regulatory factors for the growth andmigration of various cell types including vascular smooth muscle cells (VSMCs). It has also been reported that the activation of the MAP kinase family, extracellular signal regulated kinases 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK) via Src activation is important for AII-induced migration of VSMCs. MAPKs are intracellular signal-transduction pathways that have been shown to play a central role in the development of injury following ischemia in the brain and heart. MAPKs regulate gene expression, which is important in cell injury/repair and proliferation/differentiation.

PRKM2, PRKM1, p42MAPK, p41MAPK, p41, p40, p38, Mitogen-activated protein kinase 1 MAP kinase 2, Mitogen-activated protein kinase 1, MAPK2, MAPK1, Extracellular Signal Regulated Kinase 2, ERT1, ERK2, ERK, ,

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Le A, et al. (2010) Proc Natl Acad Sci U S A, 107(5):2037-42.;; Gesslein B, et al. (2010) Mol Vis,16:392-407.;; Lai EW, et al. (2004) J Invest Surg, 17:45-53.

小鼠

IgG2b kappa

重组人类MAPK1(1-360)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green).

Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK1 (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

人类

AT1A4

The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 3000. Recommended starting dilution is 1:500

人类

Cancer

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P28482

ERK, ERK 2, ERK2, ERT 1, ERT1, Extracellular Signal Regulated Kinase 2, MAPK 1, MAPK 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 1 MAP kinase 2, p38, p40, p41, p41mapk, P42MAPK, PRKM1, PRKM2, ATGA0182-10ug, ATGA0182-20ug, ATGA0182-50ug, ATGA0182-100ug, ATGA0182-250ug, ATGA0182-500ug, ATGA0182-1mg, ATGA0182-010, ATGA0182-020, ATGA0182-050, ATGA0182-100, ATGA0182-250, ATGA0182-500, ATGA0182-01M