抗体

人类SUMO 2/3抗体

ATGA0184

50微升

170美元

单克隆

小鼠

未共轭

AT10F1

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪

人类SUMO 2/3抗体

ATGA0184

NP_008868

SUMO (small ubiquitin-related modifier) is the best-characterized member of a growing family of ubiquitin-related proteins. 8-11 kDa that covalently modify various intracellular proteins. It resembles ubiquitin in its structure, its ability to be ligated to other proteins. SUMO regulates cellular function of a variety of target proteins. SUMO proteins are expressed as their precursor forms. Cleavage of the residues after the GG region of these precursors by SUMO-specific proteases in maturation is a prerequisite for subsequent sumoylation. Notably, SUMO2 and SUMO3 precursors have 96% sequence identity, and recent studies have shown protein substrates conjugated with SUMO2 or SUMO3 have similar, if not identical, biological consequences.

ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, SuMO3, SuMO2, SMT3H2, SMT3H1, SMT3B, SMT3A, SMT3 suppressor of mif two 3 homolog 2, SMT3 homolog 2, SMT 3B, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), Small ubiquitin related modifier 2, Small ubiquitin like modifier 2, Sentrin2, MGC117191, HSMT3

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Tatsuya Ii, et al. (2007) DNA Repair (Amst), 6(11): 1679-1691.;; Zheng Xu, et al. (2005) Biochem J, 386(Pt 2): 325-330.;; Melchior F. (2000) Annu Rev Cell Dev Biol, 16: 591-626.

小鼠

IgG2b kappa

重组人类SUMO2(1-93aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 免疫组化, 流式细胞仪

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human SUMO2/3 antibody (1:500) with Texas Red (Red).

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: Jurkat cell lysate;;Lane 3.: K562 cell lysateThe cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HL-60 cell lysate;;{ATGA0184-WB2.jpg}The recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human SUMO1 protein;;Lane 2.: Recombinant human SUMO2 protein;;Lane 3.: Recombinant human SUMO3 protein;;{ATGA0184-WB3.jpg}

人类

AT10F1

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of SUMO2/3 in jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human SUMO2/3 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

人类

Cancer

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P61956

HSMT 3, HSMT3, MGC117191, Sentrin 2, Sentrin2, Sentrin-2, Small ubiquitin like modifier 2, Small ubiquitin related modifier 2, Small ubiquitin-related modifier 2, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), SMT 3B, SMT3 homolog 2, SMT3 suppressor of mif two 3 homolog 2, SMT3A, SMT3B, SMT3H1, SMT3H2, SuMO 3, SuMO2, SuMO-2, SuMO3, SuMO-3, ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, ATGA0184-10ug, ATGA0184-20ug, ATGA0184-50ug, ATGA0184-100ug, ATGA0184-250ug, ATGA0184-500ug, ATGA0184-1mg, ATGA0184-010, ATGA0184-020, ATGA0184-050, ATGA0184-100, ATGA0184-250, ATGA0184-500, ATGA0184-01M

Ham JH, et al. An Immunohistochemical Study on the Expression of SUMO-2/3 in the Colorectal Carcinoma. (Soonchunhyang Med Sci. 2012){https://jsms.sch.ac.kr/journal/view.php?year=2012&vol=18&no=2&spage=95}