抗体

人类细胞角蛋白18抗体

ATGA0193

50微升

170美元

单克隆

小鼠

未共轭

AT2G3

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学

人类细胞角蛋白18抗体

ATGA0193

NP_000215

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers. Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases.

Cell proliferation inducing gene 46 protein, Cell proliferation inducing protein 46, CK 18, CYK18, Cytokeratin 18, Cytokeratin endo B, K18, Keratin 18, Keratin D, Keratin type I cytoskeletal 18, Kerd

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Moll, R, et al,. (1988) Am. J. Pathol., 132: 123-44.;; Schlimok, G, et al,. (1988) Arzneimittel-Forschung, 38: 435-7.;; Debus, E, et al,. (1984) Am. J. Pathol., 114: 121-30.

小鼠

IgG2b kappa

重组人类KRT18(79-430aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 免疫组化

ICC/IF analysis of Cytokeratin 18 in HeLa cells. The cell was stained with ATGA0193 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cytokeratin 18 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: A549 cell lysate;;Lane 3.: MCF7 cell lysate

人类

AT2G3

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human Cytokeratin 18 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

人类

细胞骨架

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P05783

Cytokeratin18, ATGA0193-10微克, ATGA0193-20微克, ATGA0193-50微克, ATGA0193-100微克, ATGA0193-250微克, ATGA0193-500微克, ATGA0193-1毫克, ATGA0193-10, ATGA0193-20, ATGA0193-50, ATGA0193-100, ATGA0193-250, ATGA0193-500, ATGA0193-1