抗体

人类CtBP1抗体

ATGA0199

50微升

170美元

单克隆

小鼠

未共轭

AT4D6

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

人类CtBP1抗体

ATGA0199

NP_001319

The C-terminal-binding protein (CtBP) was originally identified as a cellular protein that interacts with a C-terminal motif of adenovirus E1A oncoproteins. CtBP is highly conserved in higher eukaryotes. Vertebrates contain two highly homologous CtBP proteins (CtBP1 and CtBP2) while invertebrates contain a single CtBP. CtBP family proteins share significant sequence homology with D-2-hydroxy acid dehydrogenases. Also, CtBP proteins associate with various proteins and functions as a co-repressor of transcription.

C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684.

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Katsanis N, et al. (1998). Genomics 47 (2): 294-9.;; Paliwal S, et al. (March 2006). Mol. Cell. Biol. 26 (6): 2360-72.;; Chinnadurai G (2002). Mol. Cell 9 (2): 213-24.

小鼠

IgG2b kappa

重组人类CTBP1(1-440aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0199-IF1b.jpg} ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0199-IF2a.jpg} {ATGA0199-IF2b.jpg}

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: NIH3T3 cell lysate ;;Lane 3.: 293T cell lysate ;;Lane 4.: AGS cell lysate ;;Lane 5.: Raji cell lysate ;;Lane 6.: A549 cell lysate ;;Lane 7.: Jurkat cell lysate

人类

AT4D6

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500. Recommended starting dilution is 1:500

人类

信号转导

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

Q13363

CtBP1, C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684.,ATGA0199-10ug,ATGA0199-20ug,ATGA0199-50ug,ATGA0199-100ug,ATGA0199-250ug,ATGA0199-500ug,ATGA0199-1mg,ATGA0199-10,ATGA0199-20,ATGA0199-50,ATGA0199-100,ATGA0199-250,ATGA0199-500,ATGA0199-1