抗体

Human pan-RAS antibody

ATGA0203

50微升

170美元

单克隆

小鼠

未共轭

AT2G9

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

Human pan-RAS antibody

ATGA0203

NP_002515

The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes are HRAS, NRAS and KRAS.

Neuroblastoma RAS viral (v-ras) oncogene homolog, GTPase NRas, HRAS1, ALPS4, N-ras, NRAS1, NS6, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, AV095280, N ras, N ras protein part 4, Neuroblastoma RAS viral (v ras) oncogene homolog, OTTHuMP00000013879, Transforming protein N Ras, v ras neuroblastoma RAS viral oncogene homolog

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Rocks O, et al. (2006). Current Opinion in Cell Biology 18 (4): 351-7.;; Wennerberg K, et al. (March 2005). J. Cell. Sci. 118 (Pt 5): 843-6.;; Rotblat B, et al. (2008). Methods Enzymol 439: 467-89.

小鼠

IgG2a kappa

重组人类NRAS(1-186aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪

ICC/IF analysis of RAS in HeLa cells. The cell was stained with ATGA0203 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates of 293T(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (200ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human NRAS protein;;Lane 2.: Recombinant human KRAS protein;;Lane 3.: Recombinant human HRAS protein;;{ATGA0203-WB2.jpg}

人类

AT2G9

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of RAS in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

人类

信号转导

Ras Antibody detects endogenous levels of total K-Ras, H-Ras, and N-Ras(cell signaling).

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P01111

V ras neuroblastoma RAS viral oncogene homolog, Transforming protein N Ras, OTTHuMP00000013879, NS6, NRAS1, N-ras, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, Neuroblastoma RAS viral (v-ras) oncogene homolog, OTTMuSP00000023521, Neuroblastoma RAS viral (v-ras) oncogene homolog, Neuroblastoma RAS viral (v ras) oncogene homolog, N ras protein part 4, N ras, HRAS1, GTPase NRas, AV095280, ALPS4, RAS, ATGA0203-10ug, ATGA0203-20ug, ATGA0203-50ug, ATGA0203-100ug, ATGA0203-250ug, ATGA0203-500ug, ATGA0203-1mg, ATGA0203-010, ATGA0203-020, ATGA0203-050, ATGA0203-100, ATGA0203-250, ATGA0203-500, ATGA0203-01M