产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类TDG抗体
目录 :
ATGA0251
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT2F7
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类TDG抗体
目录号(SKU号) :
ATGA0251
NCBI :
AAH37557
描述 :
G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. Several bacterial proteins have strong sequence homology with this protein. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
别称 :
Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase,
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Neddermann, P ., et al. (1996) J Biol Chem 271(22): 12767-74;; Sard, L., et al. (1997) Genomics 44(2): 222-6;; Gallinari, P., et al. (1996) Nature 383(6602): 735-8
ab宿主 :
小鼠
抗体亚型 :
IgG1 kappa
免疫原 :
重组人类TDG(1-410aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF
IF Comment And Pic :
ICC/IF analysis of TDG in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TDG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0251-IF1b.jpg}
WB Comment and Pic :
The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
反应对象 :
人类
克隆编号 :
AT2F7
Ab用途 :
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:250.
抗原物种 :
人类
基因类别 :
Epigenomics (Transcription & Translation)
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
Q13569
内部搜索条款 :
TDG, Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase,ATGA0251-10ug,ATGA0251-20ug,ATGA0251-50ug,ATGA0251-100ug,ATGA0251-250ug,ATGA0251-500ug,ATGA0251-1mg,ATGA0251-10,ATGA0251-20,ATGA0251-50,ATGA0251-100,ATGA0251-250,ATGA0251-500,ATGA0251-1
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司，制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。