产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类角蛋白8/KRT8抗体
目录 :
ATGA0253
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT3D11
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类角蛋白8/KRT8抗体
目录号(SKU号) :
ATGA0253
NCBI :
NP_002264
描述 :
Keratin, type II cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that in human is encoded by the KRT8 gene. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
别称 :
KO, Keratin type II cytoskeletal 8, Keratin 8, K8, K2C8, CYK8, CK8, CARD2
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Moriya, T ., et al. (2006) Med Mol Morphol 39(1): 8-13;; Leitner, A., et al. (2012) Biophys J 103(2): 195-201
ab宿主 :
小鼠
抗体亚型 :
IgG3 kappa
免疫原 :
重组人类KRT8(1-483aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪
IF Comment And Pic :
ICC/IF analysis of KRT8 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT8 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0253-IF1b.jpg}ICC/IF analysis of KRT8 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT8 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0253-IF2a.jpg}{ATGA0253-IF2b.jpg}
WB Comment and Pic :
The cell lysate of HeLa (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: LnCap cell lysate ;;Lane 2.: PC3 cell lysate ;;Lane 3.: MCF7 cell lysate ;;Lane 4.: A549 cell lysate {ATGA0253-WB2.jpg}
反应对象 :
人类
克隆编号 :
AT3D11
Ab用途 :
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
FACS Comment and Pic :
Flow cytometry analysis of KRT8 in MCF7 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
抗原物种 :
人类
基因类别 :
细胞骨架
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
P05787
内部搜索条款 :
Type II cytoskeletal 8, KO, Keratin8, Keratin type II cytoskeletal 8, Keratin 8, Keratin, K-8, K8, K2C-8, K2C8, K2C 8, K 8, Cytokeratin8, Cytokeratin 8, CYK8, CYK 8, CK-8, CK8, CK 8, CARD-2, CARD2, CARD 2, ATGA0253-10ug, ATGA0253-20ug, ATGA0253-50ug, ATGA0253-100ug, ATGA0253-250ug, ATGA0253-500ug, ATGA0253-1mg, ATGA0253-010, ATGA0253-020, ATGA0253-050, ATGA0253-100, ATGA0253-250, ATGA0253-500, ATGA0253-01M
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司,制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。