产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类细胞角蛋白19抗体
目录 :
ATGA0255
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT13D10
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类细胞角蛋白19抗体
目录号(SKU号) :
ATGA0255
NCBI :
NP_002267
描述 :
KRT19 (type 1 cytokeratin 19) is 44kDa intermediate filament (IF) protein that is part of the Keratin family of proteins. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.
别称 :
Keratin type I cytoskeletal 19, Keratin, type I cytoskeletal 19, CK19, K19, K1CS
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Stone, M.R., et al. (2005) Mol Biol Cell 16: 4280-4293.;; Lacroix, M. (2006) Endocrine Related Cancer 13(4): 1033-1067.;; Allard W.J., et al. (2004) Clin Cancer Research 10(20): 6897-6904.
ab宿主 :
小鼠
抗体亚型 :
IgG2b kappa
免疫原 :
重组人类KRT19(1-400aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪
IF Comment And Pic :
ICC/IF analysis of KRT19 in HepG2 cells. The cell was stained with ATGA0255 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The cell lysate of MCF7 (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysate (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate {ATGA0255-WB2.jpg} The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: KRT19 Transfected 293T cell lysate {ATGA0255-WB3.jpg}
反应对象 :
人类
克隆编号 :
AT13D10
Ab用途 :
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
FACS Comment and Pic :
Flow cytometry analysis of KRT19 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
抗原物种 :
人类
基因类别 :
细胞骨架
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
P08727
内部搜索条款 :
Cytokeratin19, ATGA0255-10微克, ATGA0255-20微克, ATGA0255-50微克, ATGA0255-100微克, ATGA0255-250微克, ATGA0255-500微克, ATGA0255-1毫克, ATGA0255-10, ATGA0255-20, ATGA0255-50, ATGA0255-100, ATGA0255-250, ATGA0255-500, ATGA0255-1
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司，制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。