抗体

Human Coactosin-like Protein 1/COTL1 antibody

ATGA0300

50微升

170美元

单克隆

小鼠

未共轭

AT1D6

免疫印迹, 酶联免疫吸附测定, 流式细胞仪

Human Coactosin-like Protein 1/COTL1 antibody

ATGA0300

NP_066972

COTL1 (coactosin-like 1), also known as CLP, is a 142 amino acid cytoplasmic and cytoskeletal protein that belongs to the actin-binding proteins ADF family and coactosin subfamily. COTL1 is first identified in slime mold and is found at highest levels in kidney, placenta, lung and peripheral blood leukocytes. COTL1 is one of many proteins that participate in regulation of Actin's cytoskeleton through a calcium dependent mechanism, but has not been shown to have a direct effect on Actin depolymerization.

Coactosin like F-actin binding protein 1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, CLP

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Provost. P., et al. (2001) Biochem J 359(1): 255-263.;;Dai. H., et al. (2006) Biochim Biophys Acta 1764(11): 1688-1700.;;Esser. J., et al. (2010) Biochem J 425(1): 265-274.

小鼠

IgG2b kappa

重组人类COTL1(1-142aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 流式细胞仪

The cell lysates(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: Coactosin-like Protein 1/COTL1 Transfected 293T cell lysate;;The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: PC3 cell lysate;;Lane 2.: A549 cell lysate;;Lane 3.: U87MG cell lysate;;Lane 4.: HeLa cell lysate;;{ATGA0300-WB2.jpg}

人类

AT1D6

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of Coactosin-like Protein 1/COTL1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

人类

激素&细胞因子

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

Q14019

CLP, Coactosin like F-actin binding protein 1, Coactosin like Protein1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, Coactosin-like protein, Coactosin-like Protein 1, Coactosin-like Protein1, COTL 1, COTL1, COTL-1, FLJ43657, MGC19733, ATGA0300-10ug, ATGA0300-20ug, ATGA0300-50ug, ATGA0300-100ug, ATGA0300-250ug, ATGA0300-500ug, ATGA0300-1mg, ATGA0300-010, ATGA0300-020, ATGA0300-050, ATGA0300-100, ATGA0300-250, ATGA0300-500, ATGA0300-01M