产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
Human Glypican 4/GPC4 antibody
目录 :
ATGA0346
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT51E3
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
Human Glypican 4/GPC4 antibody
目录号(SKU号) :
ATGA0346
NCBI :
NP_001439
描述 :
The Glypican (GPC) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of Glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the initial stage of neural tube closure, suggesting that GPC4 is unlikely to play an essential role in convergent extension in the mouse.
别称 :
Glypican 4, Glypican 4, dJ900E8.1, glypican proteoglycan 4
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Ybot-Gonzalez P., et al. (2005) Dev Dyn. 233(3): 1013-1017.;; Karihaloo A., et al. (2004) Mol Cell Biol. 24(19): 8745-8752.;; Ohkawara B., et al. (2003) Development. 130(10): 2129-2138.
ab宿主 :
小鼠
抗体亚型 :
IgG2a kappa
免疫原 :
重组人类GPC4(401-529aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪
IF Comment And Pic :
ICC/IF analysis of GPC4 in 293T cells. The cell was stained with ATGA0346 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The Recombinant portein (50ng) and Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPC4 anibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : Recombinant Human GPC4 ;;Lane 2. : Ramos cell lysate
反应对象 :
人类
克隆编号 :
AT51E3
Ab用途 :
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
FACS Comment and Pic :
Flow cytometry analysis of GPC4 in 293T cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
抗原物种 :
人类
基因类别 :
Cancer
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
O75487
内部搜索条款 :
Glypican4, GPC4, ATGA0346-10微克, ATGA0346-20微克, ATGA0346-50微克, ATGA0346-100微克, ATGA0346-250微克, ATGA0346-500微克, ATGA0346-1毫克, ATGA0346-10, ATGA0346-20, ATGA0346-50, ATGA0346-100, ATGA0346-250, ATGA0346-500, ATGA0346-1
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司，制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。