产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
Human Ras antibody
目录 :
ATGA0350
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT2F8
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
Human Ras antibody
目录号(SKU号) :
ATGA0350
NCBI :
NP_203524
描述 :
All RAS protein family members belong to a class of protein called small GTPase, and are involved cellular signal transduction. RAS is 'switched on' by incoming signals, it subsequently switches on other proteins, which ultimately turn on genes involved in cell growth, differentiation and survival. Mutations in RAS genes can lead to the production of permanently activated RAS proteins. This can cause unintended and overactive signaling inside the cell, even in the absence of incoming signals. Because these signals result in cell growth and division, overactive RAS signaling can ultimately lead to cancer. The 3 Ras genes in humans (HRas, KRas, NRas) are the most common oncogenes in human cancer; mutations that permanently activate RAS are found in 20% to 25% of all human tumors and up to 90% in certain types of cancer. RAS inhibitors are being studied as a treatment for cancer, and other diseases with RAS overexpression.
别称 :
GTPase Kras, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, NS3, RASK2, GTPase Kras,K-RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS 1, NS 3
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
McGrath JP., et al. (1983) Nature. 304(5926): 501-506.;; Schubbert S., et al. (2006) Nat Genet. 38(3): 331-336.
ab宿主 :
小鼠
抗体亚型 :
IgG2b kappa
免疫原 :
重组人类KRAS(1-186aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF
IF Comment And Pic :
ICC/IF analysis of RAS in HeLa cells. The cell was stained with ATGA0350 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1. : HeLa cell lysate;;Lane 2. : HepG2 cell lysate;;Lane 3. : Ramos cell lysate;;Lane 4. : A549 cell lysate;;Lane 5. : Balb/3T3 cell lysate The Recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1. : KRAS recombinant protein (ATGP2062);;Lane 2. : HRAS recombinant protein (ATGP0516);;Lane 3. : NRAS recombinant protein (ATGP0492) {ATGA0350-WB2.jpg}
反应对象 :
人类
克隆编号 :
AT2F8
Ab用途 :
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
抗原物种 :
人类
基因类别 :
信号转导
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
P01116
内部搜索条款 :
Ras,
规格2 :
50微升
标价2(美元) :
170美元
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
salesbio@atgenglobal.com
http://www.atgenglobal.com
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司,制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。