抗体

Human Beclin 1/BECN1 antibody

ATGA0355

50微升

170美元

单克隆

小鼠

未共轭

AT45E8

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

Human Beclin 1/BECN1 antibody

ATGA0355

NP_003757

Beclin-1 is a protein that in humans is encoded by the BECN1 gene. Beclin-1 is a mammalian ortholog of the yeast autophagy-related gene (Atg) 6 and BEC-1 in the C. elegans nematode. Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy.

Beclin 1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, Beclin 1 autophagy related, ATG6, ATG6 autophagy related 6 homolog, VPS30

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liang XH., et al. (1998) J Virol. 72(11): 8586-8596.;; Takacs-Vellai K., et al. (2005) Curr Biol. 15(16): 1513-1517.

小鼠

IgG2b kappa

重组人类BECN(1-450aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪

ICC/IF analysis of BECN1 in HeLa cells. The cell was stained with ATGA0355 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The recombinant protein (10ng) and cell lysate(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BECN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human BECN1 protein;;Lane 2.: Balb/3T3 cell lysate;; The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BECN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: BECN1 Transfected 293T cell lysate{ATGA0355-WB2.jpg}

人类

AT45E8

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of BECN1 in HeLa cells. The cell was stained with ATGA0355 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

人类

Epigenomics (Transcription & Translation)

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

Q14457

Beclin1, BECN1, ATGA0355-10微克, ATGA0355-20微克, ATGA0355-50微克, ATGA0355-100微克, ATGA0355-250微克, ATGA0355-500微克, ATGA0355-1毫克, ATGA0355-10, ATGA0355-20, ATGA0355-50, ATGA0355-100, ATGA0355-250, ATGA0355-500, ATGA0355-1