抗体

人类GOT2抗体

ATGA0357

50微升

170美元

单克隆

小鼠

未共轭

AT8B11

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

人类GOT2抗体

ATGA0357

NP_002071

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. Also, GOT2 is a major participant in the malate-aspartate shuttle, which is a passage from the cytosol to the mitochondria. The two enzymes are homodimeric and show close homology. GOT2 has been seen to have a role in cell proliferation, especially in terms of tumor growth.

Aspartate aminotransferase 2 mitochondrial, Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Yang H., et al. (2015) The EMBO journal. 34(8): 1110-1125.;; Guidetti P., et al. (2007) Journal of neurochemistry. 102(1): 103-111.

小鼠

IgG3 kappa

重组人类GOT2(30-430aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪

ICC/IF analysis of GOT2 in HeLa cells. The cell was stained with ATGA0357 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The recombinant human GOT2 (50ng), cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: GOT2 Recombinant protein;;Lane 2.: Ramos cell lysate;;Lane 3.: 293T cell lysate;;Lane 4.: Liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: HepG2 cell lysate;;Lane 3.: Balb/3T3 cell lysate {ATGA0357-WB2.jpg} The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant Human GOT1;;Lane 2.: Recombinant Human GOT2 {ATGA0357-WB3.jpg}

人类

AT8B11

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of GOT2 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

人类

代谢

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P00505

GOT2, ATGA0357-10微克, ATGA0357-20微克, ATGA0357-50微克, ATGA0357-100微克, ATGA0357-250微克, ATGA0357-500微克, ATGA0357-1毫克, ATGA0357-10, ATGA0357-20, ATGA0357-50, ATGA0357-100, ATGA0357-250, ATGA0357-500, ATGA0357-1