抗体

人类甲平滑肌肌动蛋白/大动脉平滑肌肌动蛋白alpha2抗体

ATGA0358

50微升

170美元

单克隆

小鼠

未共轭

AT132D3

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

人类甲平滑肌肌动蛋白/大动脉平滑肌肌动蛋白alpha2抗体

ATGA0358

NP_001604

Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic beta- and gamma-actin proteins are referred to as non-muscle actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. Actin has major functions. Firstly, F-actin polymers form microfilaments - polar intracellular tracks for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actin is a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherens junctions. actin has a role in cell motility through polymerization and depolymerization of fibrils.

Aortic smooth muscle actin, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, Aortic smooth muscle, Intermediate form, ACTSA, ACTVS, GIG46

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Herman IM. et al. (1993) Curr Opin Cell Biol. 5(1): 48-55.;; Guo DC., et al. (2009) Am J Hum Genet. 84(5): 617-627.

小鼠

IgG1 kappa

重组人类大动脉平滑肌肌动蛋白alpha2(3-377aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪

ICC/IF analysis of ACTA2 in HeLa cells. The cell was stained with ATGA0358 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: SW480 cell lysate;;Lane 3.: HeLa cell lysate

人类

AT132D3

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of ACTA2 in Nb2-11 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

人类

Actin & Myosin Related

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P62736

Aortic smooth muscle actin, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, Aortic smooth muscle, Intermediate form, ACTSA, ACTVS, GIG46, Alpha actin 2, Alpha actin2, Cell growth inhibiting gene 46 protein, ATGA0358-10ug, ATGA0358-20ug, ATGA0358-50ug, ATGA0358-100ug, ATGA0358-250ug, ATGA0358-500ug, ATGA0358-1mg, ATGA0358-010, ATGA0358-020, ATGA0358-050, ATGA0358-100, ATGA0358-250, ATGA0358-500, ATGA0358-01M

Liu X, et al. Human aortic valve interstitial cells obtained from patients with aortic valve stenosis are vascular endothelial growth factor receptor 2 positive and contribute to ectopic calcification. (J Pharmacol Sci . 2021){https://pubmed.ncbi.nlm.nih.gov/33451756/}