抗体

人类细胞周期蛋白H抗体

ATGA0363

50微升

170美元

单克隆

小鼠

未共轭

AT3G6

免疫印迹, 酶联免疫吸附测定

人类细胞周期蛋白H抗体

ATGA0363

NP_001230

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.

CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Jeang KT., et al. (1998) J Biomed Sci. 5(1): 24-27.;; Yankulov K., et al. (1998) Current Biology. 8(13): 447-449.

小鼠

IgG2b kappa

重组人类细胞周期蛋白H(1-323aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹

The recombinant proteins (25ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human Cyclin H. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HepG2 cell lysate ;;Lane 2.: Jurkat cell lysate ;;Lane 3.: Ramos cell lysate ;;Lane 4.: Balb-3T3 cell lysate {ATGA0363-WB2.jpg}

人类

AT3G6

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000.

人类

细胞周期

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P51946

Cyclin H, CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H,ATGA0363-10ug,ATGA0363-20ug,ATGA0363-50ug,ATGA0363-100ug,ATGA0363-250ug,ATGA0363-500ug,ATGA0363-1mg,ATGA0363-10,ATGA0363-20,ATGA0363-50,ATGA0363-100,ATGA0363-250,ATGA0363-500,ATGA0363-1