抗体

磷酸甘油醛脱氢酶抗体

ATGA0394

100微升

290美元

单克隆

小鼠

未共轭

AT4E8

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

磷酸甘油醛脱氢酶抗体

ATGA0394

NP_002037

Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis in the cytoplasm, it is involved in the translational control of gene expression in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.

Glyceraldehyde-3-phosphate dehydrogenase isoform 2, G3PD, GAPD, HEL-S-162eP

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Chuang DM., et al, (2005) Annu Rev Pharmacol Toxicol. 45:269-90. ;; Mazzola JL., et al, (2002) Neurotoxicology. 23(4-5):603-9. ;; Sirover MA. (1997) J Cell Biochem. 66(2):133-40.

小鼠

IgG2b kappa

磷酸甘油醛脱氢酶来自兔肌肉

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

ICC/IF analysis of GAPDH in HeLa cells. The cell was stained with ATGA0394 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: HepG2 cell lysate;; Lane 2.: MCF7 cell lysate;; Lane 3.: Jurkat cell lysate;; Lane 4.: A549 cell lysate;; Lane 5.: K562 cell lysate;; Lane 6.: A431 cell lysate;; Lane 7.: LNCap cell lysate The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Ramos cell lysate {ATGA0394-WB2.jpg} The tissue lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Mouse Brain tissue lysate;; Lane 2.: Mouse Spleen tissue lysate;; Lane 3.: Mouse Eye tissue lysate;; Lane 4.: Mouse Stomach tissue lysate;; Lane 5.: Mouse Lung tissue lysate;; Lane 6.: Mouse Muscle tissue lysate {ATGA0394-WB3.jpg} The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Anti-GAPDH monoclonal antibody (1:100,000);;Lane 2.: Anti-GAPDH monoclonal antibody (1:50,000);;Lane 3.: Anti-GAPDH monoclonal antibody (1:10,000);;Lane 4.: Anti-GAPDH monoclonal antibody (1:1,000) {ATGA0394-WB4.jpg}

AT4E8

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

人类

神经科学

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P04406

磷酸甘油醛脱氢酶

100微升

290美元