产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类AIF/AIFM1抗体
目录 :
ATGA0411
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT22E9
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类AIF/AIFM1抗体
目录号(SKU号) :
ATGA0411
NCBI :
NP_665811
描述 :
Apoptosis-inducing factor 1, also known as AIFM1, is a mitochondrial protein that translocates to the nucleus upon induction of apoptosis. AIFM1 has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIFM1 from mitochondria, but not to block its apoptogenic activity. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy.
别称 :
Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor, AIF, CMTX4, COWCK, COXPD6, PDCD8
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Tang Y., et al. (2013) J Proteomics. 91C: 200-209.;; Kim TW., et al. (2013) Cell Death Dis. 4: e919.;;
ab宿主 :
小鼠
抗体亚型 :
IgG2a kappa
免疫原 :
重组人类AIFM1(98-609aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF
IF Comment And Pic :
ICC/IF analysis of AIFM1 in Hep3B cells. The cell was stained with ATGA0411 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1. : Jurkat cell lysate;; Lane 2. : HeLa cell lysate;; Lane 3. : Hep3B cell lysate;; Lane 4. : Raji cell lysate;; Lane 5. : K562 cell lysate;; Lane 6. : MCF7 cell lysate;; Lane 7. : CTLL2 cell lysate The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1. : Mouse heart tissue lysate;; Lane 2. : Mouse liver tissue lysate {ATGA0411-WB2.jpg}
反应对象 :
人类
克隆编号 :
AT22E9
Ab用途 :
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
抗原物种 :
人类
基因类别 :
凋亡
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
O95831
内部搜索条款 :
AIF, AIFM1, Apoptosis-inducing factor 1, mitochondrial isoform 2 precursor, Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor, CMTX4, COWCK, COXPD6, PDCD8, ATGA0411-10ug, ATGA0411-20ug, ATGA0411-50ug, ATGA0411-100ug, ATGA0411-250ug, ATGA0411-500ug, ATGA0411-1mg, ATGA0411-010, ATGA0411-020, ATGA0411-050, ATGA0411-100, ATGA0411-250, ATGA0411-500, ATGA0411-01M
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司,制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。