产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类SHMT1抗体
目录 :
ATGA0413
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT26E5
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类SHMT1抗体
目录号(SKU号) :
ATGA0413
NCBI :
AAH07979
描述 :
Serine hydroxymethyltransferase 1, also known as SHMT1, belongs to the SHMT family. This protein is cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5 10-methylene tetrahydrofolate. Also, SHMT1 preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate and inhibits.
别称 :
丝氨酸羟甲基转移酶1, CSHMT, SHMT
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Garrow TA., et al. (1993) J Biol Chem. 268(16): 11910-6.;; Perry C., et al. (2005) J Biol Chem. 280(1): 396-400. ;; Pinthong C., et al. (2014) FEBS J. 281(11): 2570-83.;;
ab宿主 :
小鼠
抗体亚型 :
IgG1 kappa
免疫原 :
重组人类SHMT1(1-483aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF
IF Comment And Pic :
ICC/IF analysis of SHMT1 in Hep3B cells. The cell was stained with ATGA0413 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SHMT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: A549 cell lysate ;;Lane 2.: HeLa cell lysate;; Lane 3.: NIH-3T3 cell lysate ;;Lane 4.: LnCaP cell lysate
反应对象 :
人类
克隆编号 :
AT26E5
Ab用途 :
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
抗原物种 :
人类
基因类别 :
代谢
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
P34896
内部搜索条款 :
SHMT1, ATGA0413-10微克, ATGA0413-20微克, ATGA0413-50微克, ATGA0413-100微克, ATGA0413-250微克, ATGA0413-500微克, ATGA0413-1毫克, ATGA0413-10, ATGA0413-20, ATGA0413-50, ATGA0413-100, ATGA0413-250, ATGA0413-500, ATGA0413-1
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司，制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。