抗体

人类S100A11抗体

ATGA0434

50微升

170美元

单克隆

小鼠

未共轭

AT20D11

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

人类S100A11抗体

ATGA0434

NP_005611

S100A11 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion, and tubulin polymerization.

S100钙结合蛋白A11, MLN70, S100C

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Sakaguchi M., et al. (2003) J Cell Biol. 163: 825-35.;; Settmacher U., et al. (2005) Int. J. Oncol. 28: 195-200.

小鼠

IgG1 kappa

重组人类S100A11(1-105aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪

ICC/IF analysis of S100A11 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : MCF7 cell lysate ;;Lane 3. : PC3 cell lysate ;;Lane 4. : THP1 cell lysate ;;Lane 5. : U87MG cell lysate ;;Lane 6. : A427 cell lysate ;;Lane 7. : SK-OV-3 cell lysate ;;Lane 8. : A549 cell lysate

人类

AT20D11

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of S100a11 in HeLa cells. The cell was stained with ATGA0434 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). Flow cytometry analysis of S100a11 in A549 cells. The cell was stained with ATGA0434 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). {2004010373111810.jpg}

人类

细胞周期

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P31949

S100A11, S100钙结合蛋白A11, MLN70, S100C, ATGA0434-10微克, ATGA0434-20微克, ATGA0434-50微克, ATGA0434-100微克, ATGA0434-250微克, ATGA0434-500微克, ATGA0434-1毫克, ATGA0434-10, ATGA0434-20, ATGA0434-50, ATGA0434-100, ATGA0434-250, ATGA0434-500, ATGA0434-1