产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类弹性蛋白抗体
目录 :
ATGA0453
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT5H8
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类弹性蛋白抗体
目录号(SKU号) :
ATGA0453
NCBI :
NP_003371
描述 :
Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastaticprogression.
别称 :
弹性蛋白, 附睾苯巴比妥蛋白113, HEL113, CTRCT30
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
John E. Eriksson.,et al. (2009) J Clin Invest. 119(7): 1763-1771. ;; Cabeen MT.,et al. (2010) Annu Rev Genet. 44: 365-392.
ab宿主 :
小鼠
抗体亚型 :
IgG1 kappa
免疫原 :
重组人类弹性蛋白(1-466aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF
IF Comment And Pic :
ICC/IF analysis of VIM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VIM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0453-IF1b.jpg}
WB Comment and Pic :
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VIM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1 : Jurkat cell lysate ;;Lane 2 : K562 cell lysate ;;Lane 3 : 293T cell lysate ;;Lane 4 : A549 cell lysate ;;Lane 5 : SK-OV-3 cell lysate ;;Lane 6 : HeLa cell lysate
反应对象 :
人类
克隆编号 :
AT5H8
Ab用途 :
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
抗原物种 :
人类
基因类别 :
神经科学
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
P08670
内部搜索条款 :
弹性蛋白, 弹性蛋白, 附睾苯巴比妥蛋白113, HEL113, CTRCT30, ATGA0453-10微克, ATGA0453-20微克, ATGA0453-50微克, ATGA0453-100微克, ATGA0453-250微克, ATGA0453-500微克, ATGA0453-1毫克, ATGA0453-10, ATGA0453-20, ATGA0453-50, ATGA0453-100, ATGA0453-250, ATGA0453-500, ATGA0453-1
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司，制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。